Comparison of BACTEC 9240 Peds Plus medium and isolator 1.5 microbial tube for detection of Brucella melitensis from blood cultures.

Journal of Clinical Microbiology
P YagupskyM Abu-Rashid

Abstract

The sensitivity and time to detection of Brucella melitensis by the BACTEC 9240 and the Isolator blood culture systems were compared in a prospective volume-controlled study. Blood sample aliquots, obtained from children with suspected brucellosis, were inoculated into a BACTEC 9240 Peds Plus bottle and into an Isolator 1.5 Microbial Tube. Overall, 122 pairs of blood samples for culture were obtained, and 28 (23%) were positive by at least one method. The BACTEC 9240 system detected all 28 positive cultures (sensitivity, 100%), and the Isolator system detected 22 positive cultures (sensitivity, 79%) (P = 0.023). Among those 22 cultures positive by both methods, 21 (95%) and 15 (68%) were found to be positive within 3 days by the BACTEC and the Isolator systems, respectively; 8 (36%) were found to be positive at least 1 day earlier by the BACTEC instrument, and the remaining 14 were found to be positive by the two systems on the same day (P = 0.045). The BACTEC 9240 blood culture system is more sensitive than the Isolator system for the detection of B. melitensis and is superior in terms of time to detection of the organism.

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Citations

Jul 5, 2011·European Journal of Clinical Microbiology & Infectious Diseases : Official Publication of the European Society of Clinical Microbiology·A A Shemesh, P Yagupsky
Aug 17, 2005·Emerging Infectious Diseases·Pablo Yagupsky, Ellen Jo Baron
Jan 5, 2000·Clinical Infectious Diseases : an Official Publication of the Infectious Diseases Society of America·G V Doern
Aug 15, 2006·Clinical Orthopaedics and Related Research·Camelia E MarculescuDouglas R Osmon
Aug 9, 2001·Transactions of the Royal Society of Tropical Medicine and Hygiene·G Perez-AvrahamK Riesenberg
Dec 6, 2020·Journal of Neurological Surgery. Part A, Central European Neurosurgery·Raul-Ciprian CovrigUlrich J Knappe
Nov 15, 2019·Clinical Microbiology Reviews·Pablo YagupskyJuan D Colmenero
Oct 19, 1999·Journal of Clinical Microbiology·P Yagupsky
May 8, 2004·Journal of Clinical Microbiology·Pablo Yagupsky
Feb 7, 2001·Journal of Clinical Microbiology·P YagupskyJ Press
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Nov 26, 2002·Clinical Microbiology and Infection : the Official Publication of the European Society of Clinical Microbiology and Infectious Diseases·Z OzkurtA Kaya

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Brucellosis is a bacterial infection caused by members of the genus brucella and remains one of the world's major zoonotic diseases. Discover the latest research on Brucellosis here.

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