Comparison of Bacterial Microbiota in Raw Mare's Milk and Koumiss Using PacBio Single Molecule Real-Time Sequencing Technology

Frontiers in Microbiology
Meng ZhangWenjun Liu

Abstract

Koumiss is a traditional fermented raw mare's milk product. It contains high nutritional value and is well-known for its health-promoting effect as an alimentary supplement. This study aimed to investigate the bacterial diversity, especially lactic acid bacteria (LAB), in koumiss and raw mare's milk. Forty-two samples, including koumiss and raw mare's milk, were collected from the pastoral area in Yili, Kazakh Autonomous Prefecture, Xinjiang Uygur Autonomous Region in China. This work applied PacBio single-molecule real-time (SMRT) sequencing to profile full-length 16S rRNA genes, which was a powerful technology enabling bacterial taxonomic assignment to the species precision. The SMRT sequencing identified 12 phyla, 124 genera, and 227 species across 29 koumiss samples. Eighteen phyla, 286 genera, and 491 species were found across 13 raw mare's milk samples. The bacterial microbiota diversity of the raw mare's milk was more complex and diverse than the koumiss. Raw mare's milk was rich in LAB, such as Lactobacillus (L.) helveticus, L. plantarum, Lactococcus (Lc.) lactis, and L. kefiranofaciens. In addition, raw mare's milk also contained sequences representing pathogenic bacteria, such as Staphylococcus succinus, Acinetobacter...Continue Reading

References

Feb 10, 2009·Veterinary Microbiology·Lotfi BounaadjaBruno Garin-Bastuji
Apr 21, 2009·Molecular Biology and Evolution·Morgan N PriceAdam P Arkin
Nov 17, 2009·Bioinformatics·J Gregory CaporasoRob Knight
Jul 22, 2010·The Journal of General and Applied Microbiology·Zhihong SunHeping Zhang
Aug 17, 2010·Bioinformatics·Robert C Edgar
Sep 10, 2010·The ISME Journal·Catherine LozuponeRob Knight
Dec 14, 2011·Current Protocols in Bioinformatics·Justin KuczynskiRob Knight
Jul 4, 2012·Nature Biotechnology·Ali BashirEric E Schadt
Jul 3, 2013·FEMS Microbiology Reviews·Lisa QuigleyPaul D Cotter
Jul 5, 2013·Genome Biology·Richard J RobertsMichael C Schatz
Aug 19, 2014·Journal of Dairy Science·Zhihong SunHeping Zhang
Sep 13, 2014·Journal of the Science of Food and Agriculture·Haiyan XuHeping Zhang
Nov 5, 2014·Advances in Experimental Medicine and Biology·Marcelo FaveriMagda Feres
Aug 9, 2016·Journal of Agricultural and Food Chemistry·Yi ZhengHeping Zhang
Dec 14, 2018·Nature Reviews. Genetics·John BeaulaurierGang Fang
Aug 10, 2019·Cell·Simon H YePardis C Sabeti

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Citations

Aug 28, 2021·Biomolecules·Gabriel DoradoPilar Hernández

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Datasets Mentioned

BETA
PRJNA646341

Methods Mentioned

BETA
electrophoresis
PCR

Software Mentioned

R
Quantitative Insights Into Microbial Ecology ( QIIME )
Basic Local Alignment Search Tool
Chimera Slayer
R ‘ ggplot2
R script
R “ pheatmap
Parallel
FastTree
RF

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