Comparison of Bacterial Swab Samples Given Different Storage Treatments 1

Journal of Food Protection
N G MarriottD R Lee

Abstract

Six paired sides of U.S. Good beef were swabbed to obtain microbial samples. Half of the beef sides were stored at an elevated temperature (25 C) for 8 h with subsequent storage for 12 h at 1 C before sampling, whereas the counterpart sides were stored the entire period at 1 C. Samples from adjacent areas were taken so that a comparison could be made of 0.03 M phosphate buffer and 0.1% peptone broth as diluents for swab samples. To evaluate the effect of storage time, swab samples were stored at 5 C for 1, 24, 48, 72, and 120 h before being transferred to Standard Plate Count Agar and subsequently incubated at 25 C. Results revealed that no differences (P>.05) existed between the effectiveness of a phosphate buffer solution and peptone broth as diluents for swab samples. Storage of swab samples at 1 C resulted in recovery of fewer microorganisms at 24, 48, and 72 h.

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