Comparison of base inclination of ribo-GC and deoxyribo-GC polymers, and synthesis of poly(rGrC)-poly(rGrC)

Biopolymers
X Jin, W C Johnson

Abstract

The inclination angle between the base normal and the helix axis, and the axes around which the bases incline, are measured for ribo-GC polymers in buffer by using flow linear dichroism (LD), and compared to measurements for deoxyribo-GC polymers in buffer and under dehydrating conditions. A new method is designed to synthesize poly(rGrC)-poly(rGrC), which is not available commercially, in large quantities. The LD of this RNA reveals inclination angles that are similar to the B-form DNA in buffer, although the axes are different. The CD of poly(dGdC)-poly(dGdC) under the dehydrating conditions is similar to poly(rGrC)-poly(rGrC), indicating it is in the A form, and the LD gives larger inclination angles than either the B form or the corresponding RNA. Poly(dG)-poly(dC) is in the A form in buffer. Comparison among poly(rG)-poly(rC) in buffer, and poly(dG)-poly(dC) in buffer under dehydrating conditions, reveals similar inclination angles and axes, although the LD shows that the DNA has the largest inclination angles. Except for poly(rGrC)-poly(rGrC), which has a unique reduced dichroism, all the axes for G are similar, as are the axes for C.

References

Apr 1, 1979·Biopolymers·C A SprecherW C Johnson
Jun 8, 1977·Journal of the American Chemical Society·L B Clark
Mar 25, 1976·Nature·F M Pohl
Jul 11, 1985·Nucleic Acids Research·J H RiazanceI Tinoco
Jan 1, 1987·Annual Review of Biophysics and Biophysical Chemistry·D J PatelD Hare
Mar 1, 1988·Proceedings of the National Academy of Sciences of the United States of America·H Y Mei, J K Barton
Mar 1, 1987·Proceedings of the National Academy of Sciences of the United States of America·E Charney, H H Chen
Dec 22, 1986·Nucleic Acids Research·V P AntaoR L Ratliff
Jan 1, 1985·Archives of Biochemistry and Biophysics·K HallM J Chamberlin
Aug 27, 1985·Biochemistry·S P Edmondson, W C Johnson
Oct 4, 1972·Nature: New Biology·S Bram, P Tougard
Sep 15, 1974·Journal of Molecular Biology·S Arnott, E Selsing
Dec 4, 1973·Biochemistry·J C GirodM F Maestre
Aug 1, 1981·Proceedings of the National Academy of Sciences of the United States of America·J C SutherlandP Z Takacs
Sep 1, 1982·Biopolymers·G C Causley, W C Johnson
Oct 15, 1980·Journal of Molecular Biology·A G LeslieR L Ratliff

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