Comparison of base-line and chemical-induced transcriptomic responses in HepaRG and RPTEC/TERT1 cells using TempO-Seq.

Archives of Toxicology
Alice LimoncielPaul Jennings

Abstract

The utilisation of genome-wide transcriptomics has played a pivotal role in advancing the field of toxicology, allowing the mapping of transcriptional signatures to chemical exposures. These activities have uncovered several transcriptionally regulated pathways that can be utilised for assessing the perturbation impact of a chemical and also the identification of toxic mode of action. However, current transcriptomic platforms are not very amenable to high-throughput workflows due to, high cost, complexities in sample preparation and relatively complex bioinformatic analysis. Thus, transcriptomic investigations are usually limited in dose and time dimensions and are, therefore, not optimal for implementation in risk assessment workflows. In this study, we investigated a new cost-effective, transcriptomic assay, TempO-Seq, which alleviates the aforementioned limitations. This technique was evaluated in a 6-compound screen, utilising differentiated kidney (RPTEC/TERT1) and liver (HepaRG) cells and compared to non-transcriptomic label-free sensitive endpoints of chemical-induced disturbances, namely phase contrast morphology, xCELLigence and glycolysis. Non-proliferating cell monolayers were exposed to six sub-lethal concentrations...Continue Reading

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Citations

Oct 20, 2018·Frontiers in Genetics·Elias ZgheibPaul Jennings
Feb 12, 2020·Genes and Environment : the Official Journal of the Japanese Environmental Mutagen Society·Julie K BuickCarole L Yauk
Jul 14, 2020·Frontiers in Genetics·Pierre R BushelScott S Auerbach
Apr 11, 2020·Toxicological Sciences : an Official Journal of the Society of Toxicology·Maria T BaltazarCarl Westmoreland
Oct 23, 2020·Bioinformatics and Biology Insights·Deepak MavRichard S Paules
Feb 5, 2021·Toxicological Sciences : an Official Journal of the Society of Toxicology·Joshua A HarrillRichard S Judson
Feb 26, 2021·Toxicology in Vitro : an International Journal Published in Association with BIBRA·Sara WellensMaxime Culot
Jun 8, 2021·Toxicology in Vitro : an International Journal Published in Association with BIBRA·Leonie F H Fransen, Martin O Leonard
Aug 6, 2021·Toxicology in Vitro : an International Journal Published in Association with BIBRA·Pranika SinghThomas E Exner

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Methods Mentioned

BETA
PCR
TempO-Seq

Software Mentioned

ToxPi
Excel
DESeq2
Seq
Bowtie aligner
TempO

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