Comparison of Biomarker Assays for EGFR: Implications for Precision Medicine in Patients with Glioblastoma.

Clinical Cancer Research : an Official Journal of the American Association for Cancer Research
Andrew B LassmanMartin J van den Bent

Abstract

Patients with glioblastoma (GBM) have a poor prognosis and are in desperate need of better therapies. As therapeutic decisions are increasingly guided by biomarkers, and EGFR abnormalities are common in GBM, thus representing a potential therapeutic target, we systematically evaluated methods of assessing EGFR amplification by multiple assays. Specifically, we evaluated correlation among fluorescence in situ hybridization (FISH), a standard assay for detecting EGFR amplification, with other methods.Experimental Design: Formalin-fixed, paraffin-embedded tumor samples were used for all assays. EGFR amplification was detected using FISH (N = 206) and whole-exome sequencing (WES, N = 74). EGFR mRNA expression was measured using reverse transcription-polymerase chain reaction (RT-PCR, N = 206) and transcriptome profiling (RNAseq, N = 64). EGFR protein expression was determined by immunohistochemistry (IHC, N = 34). Significant correlations among various methods were determined using Cohen's kappa (κ = 0.61-0.80 defines substantial agreement) or R 2 statistics. EGFR mRNA expression levels by RNA sequencing (RNAseq) and RT-PCR were highly correlated with EGFR amplification assessed by FISH (κ = 0.702). High concordance was also observ...Continue Reading

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Citations

Oct 19, 2019·Acta Neuropathologica Communications·Arne ChristiansChristian Hartmann
Aug 27, 2021·Journal of Clinical Oncology : Official Journal of the American Society of Clinical Oncology·Suresh S RamalingamJulien Mazieres

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