Comparison of biotyping, ribotyping, and pulsed-field gel electrophoresis for investigation of a common-source outbreak of Burkholderia pickettii bacteremia.

Journal of Clinical Microbiology
H ChetouiP De Mol

Abstract

Over a 3-month period, six immunocompromised patients developed one or more episodes of Burkholderia pickettii bacteremia and/or catheter infection. Vials of a commercially available, "sterile" saline for injection which had been used for flushing the patients' indwelling intravenous devices were implicated as the common source of the organisms. No further cases were diagnosed once the use of this saline was discontinued. Twenty-six isolates, including 9 outbreak-related strains from case patients and contaminated saline as well as 17 control strains, were tested comparatively by biotyping, ribotyping with EcoRI and HindIII, and pulsed-field gel electrophoresis (PFGE) with SpeI. Macrorestriction analysis revealed nine PFGE groups and was more discriminating than ribotyping (seven ribotypes) and biotyping (two biovars). Among the outbreak-related isolates, one B. pickettii type was found by the three typing methods. Furthermore, PFGE was useful for subdividing ribotypes and for distinguishing isolates involved in the outbreak from all epidemiologically unrelated strains.

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Citations

Jun 7, 2006·Infection Control and Hospital Epidemiology : the Official Journal of the Society of Hospital Epidemiologists of America·F BarbutN C Gorin
Apr 3, 2012·Anesthesia and Analgesia·Randy W LoftusMark P Yeager
Feb 5, 2015·Case Reports in Infectious Diseases·Joseph OrmeNegin N Blattman
Nov 20, 1998·The American Journal of Gastroenterology·K H YuenW F Ng
May 4, 2005·Spinal Cord·H SudoA Minami
Sep 24, 2013·European Journal of Clinical Microbiology & Infectious Diseases : Official Publication of the European Society of Clinical Microbiology·M P Ryan, C C Adley
Apr 20, 2001·Acta Clinica Belgica·G T'SjoenD Vogelaers
Oct 27, 2004·Antimicrobial Agents and Chemotherapy·Delphine GirlichPatrice Nordmann
Sep 2, 2006·Microbiology·Delphine GirlichPatrice Nordmann
Oct 19, 1999·Clinical Infectious Diseases : an Official Publication of the Infectious Diseases Society of America·J A LabarcaW R Jarvis
May 21, 2019·Emerging Infectious Diseases·Helena M B Seth-SmithAdrian Egli
Dec 6, 2006·The Journal of Hospital Infection·R-P Vonberg, P Gastmeier

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