Comparison of bone marrow cells harvested from various bones of cynomolgus monkeys at various ages by perfusion or aspiration methods: a preclinical study for human BMT

Stem Cells
Taketoshi KushidaS Ikehara

Abstract

Using cynomolgus monkeys, we have previously established a new method for harvesting bone marrow cells (BMCs) with minimal contamination of the BMCs with T cells from the peripheral blood. We originally conducted this new "perfusion method" in the long bones (the humerus, femur, and tibia) of cynomolgus monkeys. Here, we apply the perfusion method to obtain BMCs from the ilium of cynomolgus monkeys, since BMCs are usually collected from the ilium by the conventional aspiration method in humans. The perfusion method consists of two approaches: transverse iliac perfusion and longitudinal iliac perfusion. BMCs harvested by the perfusion method from the long bones and ilium were compared with those collected from the ilium by the aspiration method. The contamination of BMCs with peripheral blood, determined by the frequencies of CD4+ and CD8+ T cells, was significantly lower in BMCs obtained from the ilium or long bones by the perfusion method (CD4+ plus CD8+ T cells <4%) than in those obtained by the iliac aspiration method (CD4+ plus CD8+ T cells >20%). However, the numbers of immature myeloid cells, such as myeloblasts, promyelocytes, myelocytes, and metamyelocytes, were higher in BMCs obtained by the iliac perfusion method than...Continue Reading

References

Mar 7, 1991·The New England Journal of Medicine·J L Ferrara, H J Deeg
Jan 1, 1991·The Journal of the Florida Medical Association·W F Cassano
Oct 1, 1970·Blood·E D Thomas, R Storb
Mar 4, 1993·The New England Journal of Medicine·N A KernanJ McCullough
Jul 3, 1999·The New England Journal of Medicine·E D Thomas
Mar 24, 2000·Clinical Immunology : the Official Journal of the Clinical Immunology Society·D H Sachs
Nov 10, 2000·Stem Cells·T KushidaS Ikehara
Nov 18, 2000·Bone Marrow Transplantation·S LeeS Ikehara

❮ Previous
Next ❯

Citations

Jun 26, 2008·Annals of Hematology·Junko KatoSusumu Ikehara
Jul 18, 2009·Clinical Reviews in Allergy & Immunology·Susumu Ikehara
Oct 6, 2007·Immunologic Research·Susumu Ikehara
May 21, 2011·International Journal of Hematology·Shinichiro MoriSusumu Ikehara
Jun 3, 2011·Veterinary Clinical Pathology·William J ReaganUNKNOWN Bone Marrow Working Group of ASVCP/STP
Oct 4, 2011·American Journal of Veterinary Research·Masahiko SatoHajime Tsujimoto
Sep 20, 2011·Best Practice & Research. Clinical Haematology·Susumu Ikehara
Sep 18, 2009·Annals of the New York Academy of Sciences·Susumu Ikehara
Aug 30, 2008·Journal of Orthopaedic Research : Official Publication of the Orthopaedic Research Society·Masayuki UmedaHirokazu Iida
Apr 12, 2005·The Journal of Surgical Research·Mona G FloresDominic C Borie
May 18, 2004·Experimental Hematology·Eliane Gluckman
Jan 1, 2010·Advances in Virology·Sansanee NoisakranGuey Chuen Perng
Jul 19, 2013·The Journal of Bone and Joint Surgery. American Volume·Christopher F HyerTerrence M Philbin
Sep 1, 2004·Molecular Therapy : the Journal of the American Society of Gene Therapy·Kyoji UedaKeiya Ozawa
Jul 1, 2017·SICOT-J·Ersin KuyucuMurat Bülbül
Feb 9, 2011·Toxicologic Pathology·William J ReaganUNKNOWN Bone Marrow Working Group of ASVCP/STP
Mar 24, 2011·Expert Review of Clinical Immunology·Susumu Ikehara

❮ Previous
Next ❯

Related Concepts

Related Feeds

Allogenic & Autologous Therapies

Allogenic therapies are generated in large batches from unrelated donor tissues such as bone marrow. In contrast, autologous therapies are manufactures as a single lot from the patient being treated. Here is the latest research on allogenic and autologous therapies.