Comparison of bronchoalveolar lavage fluid obtained from Mannheimia haemolytica-inoculated calves with and without prior treatment with the selectin inhibitor TBC1269

American Journal of Veterinary Research
Jessica M CaverlyMark R Ackermann

Abstract

To determine effects of selectin inhibitor TBC1269 on neutrophil infiltration, and neutrophil-associated injury during pneumonia induced by Mannheimia haemolytica and concentration of antimicrobial anionic peptide (AAP) in bronchoalveolar lavage fluid (BALF) as well as antimicrobial activity of BALF from healthy (control) neonatal calves, neonatal calves with M haemolytica-induced pneumonia, neonatal calves with prior treatment with TBC1269, and adult cattle. Eighteen 1- to 3-day-old calves and 9 adult cattle. Calves were inoculated with M haemolytica or pyrogen-free saline (0.14M NaCl) solution into the right cranial lung lobe, and BALF was collected 2 or 6 hours after inoculation. Thirty minutes before and 2 hours after inoculation, 4 calves received TBC1269. The BALF collected from 9 adult cattle was used for comparison of BALF AAP concentration and antimicrobial activity. Protein concentration and neutrophil differential percentage and degeneration in BALF were determined. An ELISA and killing assay were used to determine BALF AAP concentration and antimicrobial activity, respectively. Total protein concentration was significantly decreased in BALF from calves receiving TBC1269. Similar concentrations of AAP were detected i...Continue Reading

References

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Citations

Nov 7, 2003·International Journal of Antimicrobial Agents·Kim A BrogdenBrian F Tack
Jan 31, 2002·Journal of Veterinary Internal Medicine·Z A RadiM R Ackermann
Jan 12, 2011·Microbial Pathogenesis·Nicole A AulikCharles J Czuprynski
Jun 26, 2020·Frontiers in Immunology·Kristen A ByrneJodi L McGill

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