Comparison of c-jun, junB, and junD mRNA expression and protein in the rat dorsal root ganglia following sciatic nerve transection

Journal of Neuroscience Research
M De LeónM A Ruda

Abstract

The present study was designed to compare the expression of the Jun family of protooncogenes following nerve injury. Adult rats were anesthetized and the sciatic nerve transected. Dorsal root ganglia (DRG) at 1, 2, 3, and 7 days after nerve transection were collected, their total RNA extracted, and Northern blots performed using 32P-labeled oligonucleotide probes. The constitutive expression of c-jun mRNA was very low in DRG. Induction of c-jun mRNA was observed by day 1 after nerve transection, with a sixfold peak at 3 days and a twofold induction still present by day 7. The constitutive expression of junB mRNA was also low in the DRG, and sciatic nerve transection produced only a modest induction (1.7-fold by day 3) in the DRG ipsilateral to the nerve cut. junD mRNA was constitutively expressed at high levels in the DRG, and its level of expression did not in the DRG, and its level of expression did not change after sciatic nerve transection. Immunocytochemistry studies demonstrated a pattern of c-Jun, JunB, and JunD immunoreactivity (IR) associated with the cell nuclei of DRG neurons. c-Jun IR was found at very low levels in the undamaged contralateral DRG neurons, but sciatic nerve transection dramatically increased the num...Continue Reading

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Citations

Oct 3, 2003·Trends in Neurosciences·Florence M Bareyre, Martin E Schwab
Sep 1, 2002·Expert Review of Neurotherapeutics·Hai-Ying M Cheng, Josef M Penninger
Mar 23, 2004·The Journal of Histochemistry and Cytochemistry : Official Journal of the Histochemistry Society·Wiphawi HipkaeoShoichi Iseki
Dec 25, 2003·The Journal of Comparative Neurology·Li-Ya Qiao, Margaret A Vizzard
Dec 22, 2004·The Journal of Comparative Neurology·Li-Ya Qiao, Margaret A Vizzard

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