PMID: 9524072Apr 2, 1998Paper

Comparison of calpains from rabbit, monkey, human and rat

Biological Chemistry
S KawashimaK Asaoka

Abstract

Two isozymes of calpain, mu-calpain and m-calpain, were purified from rabbit, monkey, human and rat tissues to homogeneity and the apparent molecular masses of the large and small subunits of each calpain species were compared directly. While the molecular masses of the small subunits were the same (28 kDa), those of the large subunits were different depending on the calpain type and animal species: Rabbit mu (79 kDa), rabbit m (75 kDa), monkey mu (79 kDa), monkey m (74 kDa), human mu (78 kDa), human m (73 kDa), rat mu (75 kDa), and rat m (74 kDa). Ca2+-sensitivity of monkey mu-calpain was lower than that of rabbit mu-calpain, but m-calpains from rabbit and monkey shared a similar Ca2+-dependency. Immunoreactivities of rabbit, monkey and rat m-calpains towards anti-rabbit m-calpain monoclonal antibodies were different depending on the antibody species, showing the existence of common and different antigenic sites in these three m-calpains. While monkey mu-calpain still showed weak cross-reactivity with anti-rabbit mu-calpain monoclonal antibodies, rat mu-calpain failed to react with any antibody examined, except for the monoclonal 1D10A7 which reacted with mu- and m-calpains from any animal species. Peptides generated by V8 pro...Continue Reading

References

Oct 29, 1987·Biochemical and Biophysical Research Communications·M HayashiS Kawashima
May 12, 1988·Biochimica Et Biophysica Acta·S KawashimaK Imahori
Jan 1, 1982·Analytical Biochemistry·R HawkesJ Gordon
Apr 18, 1994·FEBS Letters·H SorimachiK Suzuki

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Citations

Sep 13, 2005·Biochemical Pharmacology·H WeberP Schuff-Werner
May 11, 2004·American Journal of Physiology. Gastrointestinal and Liver Physiology·Heike WeberPeter Schuff-Werner

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