Comparison of Cell Arrays and Multi-Well Plates in Microscopy-Based Screening

High-throughput
Ann-Kristin BeckerVytaute Starkuviene

Abstract

Multi-well plates and cell arrays enable microscopy-based screening assays in which many samples can be analysed in parallel. Each of the formats possesses its own strengths and weaknesses, but reference comparisons between these platforms and their application rationale is lacking. We aim to fill this gap by comparing two RNA interference (RNAi)-mediated fluorescence microscopy-based assays, namely epidermal growth factor (EGF) internalization and cell cycle progression, on both platforms. Quantitative analysis revealed that both platforms enabled the generation of data with the appearance of the expected phenotypes significantly distinct from the negative controls. The measurements of cell cycle progression were less variable in multi-well plates. The result can largely be attributed to higher cell numbers resulting in less data variability when dealing with the assay generating phenotypic cell subpopulations. The EGF internalization assay with a uniform phenotype over nearly the whole cell population performed better on cell arrays than in multi-well plates. The result was achieved by scoring five times less cells on cell arrays than in multi-well plates, indicating the efficiency of the cell array format. Our data indicate ...Continue Reading

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Citations

Jan 27, 2019·SLAS Discovery·Vytaute StarkuvieneHolger Erfle

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Methods Mentioned

BETA
gene knock-down
transfection
gene knock-out
Assay

Software Mentioned

KNIME
MATLAB
R stats package
KNIME Image Processing Plugin ( KNIP )
Micromanager
R
ImageJ
Fiji

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