Comparison of cell membrane damage induced by the therapeutic ultrasound on human breast cancer MCF-7 and MCF-7/ADR cells
Abstract
The aim of this study was to compare the cell membrane damage induced by ultrasound at different intensities between MCF-7/ADR cells and MCF-7 cells. Tumor cells in the culture dishes (35 mm diameter) were exposed to planner ultrasound at intensities range from 0.25 W/cm(2) to 0.75 W/cm(2) for 60s. The viability of cells was determined by 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl tetrazolium bromide (MTT) assay and Guava Viacount assay. The cell membrane integrity was estimated by flow cytometry using propidium iodide (PI) staining and cellular uptake of fluorescein isothiocyanate-dextran (FD500). The membrane lipid peroxidation and membrane fluidity were also specially compared between two cell lines in this paper using spectrophotometry. Ultrastructural alterations on membrane surface were observed by scanning electron microscopy. The ultrasound produced cytotoxicity in both cell lines increased with the irradiation intensity increased from 0.25 W/cm(2) to 0.75 W/cm(2). Cell membrane permeability and the level of lipid peroxidation were remarkably enhanced after ultrasound application. In addition, relatively severe cell damage was observed under scanning electron microscopy after 0.75 W/cm(2) ultrasound treatment. Ultrasound...Continue Reading
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