Comparison of changes in endothelial adhesion molecule expression following UVB irradiation of skin and a human dermal microvascular cell line (HMEC-1)

Photodermatology, Photoimmunology & Photomedicine
L E RhodesP S Friedmann

Abstract

We have assessed the pattern of dermal endothelial adhesion molecule expression following broadband UVB irradiation in vivo and in vitro. Skin biopsies were taken from 4 human volunteers at baseline and at 4, 8 and 24 h post-irradiation with 2.5 minimal erythema doses of UVB. Sections were stained immunohistochemically for E-selectin, intercellular adhesion molecule-1 (ICAM-1), vascular cell adhesion molecule-1 (VCAM-1). CD31 and neutrophil elastase. The effect of direct UVB irradiation on E-selectin, ICAM-1 and VCAM-1 was examined in a human dermal microvascular endothelial cell line, HMEC-1. Cultured HMEC-1 were irradiated with 2.5-40 mJ/cm2 of UVB, and assessed for adhesion molecule expression by immunofluorescence microscopy and fluorescence-activated cell sorter analysis. In vivo, E-selectin was minimally expressed on EC at baseline and was induced by 4 h following irradiation, P < 0.01. ICAM-1 was moderately expressed at baseline and appeared mildly induced at 24 h, although this did not reach statistical significance. VCAM-1 was weakly expressed in unirradiated skin while CD31 was moderately expressed, but neither was induced by UVB irradiation. A significant neutrophilic infiltrate appeared by 8 h and was maximal at 24 ...Continue Reading

References

Feb 1, 1979·The Journal of Investigative Dermatology·W H EaglsteinN Mizuno
Dec 1, 1992·The Journal of Investigative Dermatology·E W AdesT J Lawley
Jan 26, 1991·Lancet·J N BarkerB J Nickoloff
Feb 1, 1991·The British Journal of Dermatology·R W GrovesD M MacDonald
Sep 1, 1991·Proceedings of the National Academy of Sciences of the United States of America·U H von AndrianE C Butcher
Aug 2, 1990·Nature·T A Springer
Jan 1, 1988·The British Journal of Dermatology·J L HawkC A Holden
Feb 1, 1987·The British Journal of Dermatology·B L Diffey, A M Oakley
Jul 1, 1966·Photochemistry and Photobiology·M A EverettR L Olson
Oct 1, 1981·Journal of the American Academy of Dermatology·B A GilchrestM C Mihm
Feb 1, 1994·Current Opinion in Immunology·G P Downey
Aug 1, 1993·The Journal of Experimental Medicine·W A MullerD M Phillips
Mar 1, 1993·Clinical and Experimental Immunology·P S FriedmannP M Johnson

❮ Previous
Next ❯

Citations

Aug 17, 2002·International Journal of Radiation Oncology, Biology, Physics·Franz-Josef ProttJosef Piffko
May 12, 2001·European Journal of Oral Sciences·J HandschelU Joos
Sep 16, 1999·International Journal of Radiation Oncology, Biology, Physics·J HandschelU Joos
Nov 20, 2004·Journal of Thrombosis and Haemostasis : JTH·M-H GauglerJ Aigueperse

❮ Previous
Next ❯

Related Concepts

Related Feeds

Adhesion Molecules in Health and Disease

Cell adhesion molecules are a subset of cell adhesion proteins located on the cell surface involved in binding with other cells or with the extracellular matrix in the process called cell adhesion. In essence, cell adhesion molecules help cells stick to each other and to their surroundings. Cell adhesion is a crucial component in maintaining tissue structure and function. Discover the latest research on adhesion molecule and their role in health and disease here.

Related Papers

European Archives of Oto-rhino-laryngology : Official Journal of the European Federation of Oto-Rhino-Laryngological Societies (EUFOS) : Affiliated with the German Society for Oto-Rhino-Laryngology - Head and Neck Surgery
Young-Hoon JooMin-Sik Kim
Radiotherapy and Oncology : Journal of the European Society for Therapeutic Radiology and Oncology
Stefan Schultze-MosgauFranz Rödel
Radiotherapy and Oncology : Journal of the European Society for Therapeutic Radiology and Oncology
Cornelia Katharina Mueller, Stefan Schultze-Mosgau
© 2021 Meta ULC. All rights reserved