Comparison of chemical and cell-based antioxidant methods for evaluation of foods and natural products: generating multifaceted data by parallel testing using erythrocytes and polymorphonuclear cells

Journal of Agricultural and Food Chemistry
Dana HonzelGitte S Jensen

Abstract

The objective of this study was to compare three tests frequently used for evaluation of antioxidant potential in natural products: (1) oxygen radical absorbance assay (ORAC), (2) cell-based antioxidant protection in an erythrocyte model (CAP-e), and (3) reactive oxygen species formation in polymorphonuclear cells (ROS PMN). The methods were applied to four natural products, all containing antioxidants capable of entering and protecting cells in the CAP-e assay. The magnitude of this effect was not directly correlated to the ORAC value of each product. Furthermore, the products showed different effects in the ROS PMN assay. Acai provided strong inhibition of ROS formation, indicating anti-inflammatory properties. In contrast, Immunel and EpiCor mildly enhanced ROS formation, suggesting activation of the innate immune response. HA Joint Formula showed a complex, nonlinear dose-response in the ROS PMN assay. This illustrates that complex natural products may have similar antioxidant properties but different effects on human cells. Cell-based antioxidant protection is addressed best in the CAP-e assay, since some natural products contain compounds that may provoke cellular signaling in other cell types. The PMN cell type is a usef...Continue Reading

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Citations

Sep 8, 2012·Journal of Agricultural and Food Chemistry·Federica CheliAntonella Baldi
Apr 17, 2013·Journal of Agricultural and Food Chemistry·Martin D DimitrovPencho V Venkov
Jan 8, 2013·Journal of Medicinal Food·Kathleen F BensonGitte S Jensen
Dec 1, 2011·Journal of Clinical Biochemistry and Nutrition·Joyce Ferreira da Costa GuerraMaria Lúcia Pedrosa
May 2, 2012·Evidence-based Complementary and Alternative Medicine : ECAM·Malkanthi EvansLarry E Robinson
Jul 26, 2012·Oxidative Medicine and Cellular Longevity·C WidénK Rumpunen
Oct 2, 2014·Journal of Medicinal Food·Gitte S JensenDavid Ager
Aug 20, 2015·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Laura BraudJean-Michel Maixent
Jan 24, 2013·Analytica Chimica Acta·Camilo López-Alarcón, Ana Denicola
Jan 2, 2010·Regulatory Toxicology and Pharmacology : RTP·A ClewellA G Schauss
Dec 15, 2015·Chemico-biological Interactions·Karolina Malczewska-JaskółaLucyna Mrówczyńska
Mar 16, 2012·Journal of Food Science·Federica Cheli, Antonella Baldi
Dec 17, 2014·Free Radical Research·R Amorati, L Valgimigli
Aug 12, 2014·Journal of Medicinal Food·Gitte S JensenKathleen F Benson
Jan 10, 2012·International Journal of Toxicology·Alexander G SchaussAmy Clewell
Feb 12, 2019·Journal of Peptide Science : an Official Publication of the European Peptide Society·Helena TauteAnabella R M Gaspar
Nov 4, 2020·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Kristina RadićDubravka Vitali Čepo
Feb 25, 2014·Journal of Agricultural and Food Chemistry·Xin XuWendong Mao
May 2, 2018·Biomedicine & Pharmacotherapy = Biomédecine & Pharmacothérapie·Satish KanharAjay Kumar Mahapatra

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