Comparison of chicken gut colonisation by the pathogens Campylobacter jejuni and Clostridium perfringens by real-time quantitative PCR

Molecular and Cellular Probes
Beate SkånsengKnut Rudi

Abstract

We compared the colonisation of the chicken gut by the two important pathogens Campylobacter jejuni (frequent food-borne pathogen) and alpha-toxin gene containing Clostridium perfringens (causative agent of necrotic enteritis in chickens) using a new high-throughput automated DNA purification method for microbial biodiversity analyses. The method gave high reproducibility (standard deviation of 1.1 C(T)-values for a universal 16S rDNA real-time PCR), and inhibition was observed in only 0.9% of the individual DNA purifications (n = 753). We analysed 253 randomly collected chicken caecal samples (sampled in 2001 and 2003) from Norwegian chicken flocks by real-time quantitative PCR. Our results showed positive correlation (P = 0.009) in chicken caecal colonisation between C. jejuni and Cl. perfringens. We also found that there was a significant underrepresentation (P = 0.008) of chickens containing high levels of Cl. perfringens and low levels of C. jejuni. This indicates a possible interaction between these bacteria. Potential interaction between pathogens and other bacteria in the gut will certainly be important research fields in the future. As demonstrated here, the development of new tools for high-throughput analyses will be...Continue Reading

References

Apr 1, 1994·Molecular and Cellular Probes·R F WangC E Cerniglia
Apr 22, 1999·Trends in Microbiology·L PetitM R Popoff
Jul 9, 1999·FEMS Immunology and Medical Microbiology·A Løvland, M Kaldhusdal
Nov 5, 1999·Clinics in Laboratory Medicine·P I Fields, D L Swerdlow
Aug 29, 2001·FEMS Microbiology Reviews·P M VignaisJ Meyer
Jan 17, 2002·Proceedings of the National Academy of Sciences of the United States of America·Tohru ShimizuHideo Hayashi
Jun 1, 2002·Applied and Environmental Microbiology·Janet E HillSean M Hemmingsen
Dec 3, 2002·Science·Jonathan W Olson, Robert J Maier
Aug 9, 2003·Journal of Clinical Microbiology·Julia H KneppAlexandra Valsamakis
Nov 7, 2003·Applied and Environmental Microbiology·Jiangrang LuMargie D Lee
Apr 20, 2004·Journal of Bacteriology·Silvia G AcinasMartin F Polz
May 25, 2004·Preventive Veterinary Medicine·Kari GraveKnut Flatlandsmo
Jan 26, 2005·Biochemical Society Transactions·R J Maier
Aug 5, 2006·Anaerobe·R W TitballA K Basak

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Citations

Jul 22, 2008·Applied and Environmental Microbiology·Stephanie A BullFrieda Jorgensen
Nov 21, 2007·PLoS Pathogens·Beate SkånsengKnut Rudi
Sep 22, 2010·International Journal of Food Microbiology·Martina FrickerMonika Ehling-Schulz
Jun 16, 2009·International Journal of Food Microbiology·Bjørn Christian SchirmerSolveig Langsrud
Jul 3, 2007·International Journal of Food Microbiology·K RudiT Naes
Mar 21, 2009·Journal of Applied Microbiology·B C SchirmerS Langsrud
May 21, 2010·Environmental Microbiology·Pål TrosvikNils Chr Stenseth
Jul 14, 2011·Clinical and Experimental Allergy : Journal of the British Society for Allergy and Clinical Immunology·O StorrøR Johnsen
Oct 17, 2015·Avian Diseases·Orhan SahinQijing Zhang
Jul 4, 2012·Veterinary Microbiology·Knut RudiMichael R F Lee
Apr 4, 2008·The ISME Journal·Pål TrosvikNils C Stenseth
May 17, 2018·Avian Pathology : Journal of the W.V.P.A·Wageha A AwadMichael Hess
Jun 11, 2021·Frontiers in Veterinary Science·Robert Valeris-ChacinRandall S Singer

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