Abstract
(I) There is little discrimination between cholesterol and the plant sterol sitosterol in the uptake at the brush border membrane (BBM). (II) This difference cannot account for the marked discrimination between cholesterol and sitosterol observed in the absorption of these two sterols by the small-intestinal epithelium. (III) This discrimination occurs during intracellular processing involving the esterification and incorporation into lipoprotein particles of the two sterols. This conclusion is based on a comparative study of sterol uptake by brush border membrane vesicles (BBMV) and sterol absorption by Caco-2 cells. (IV) The uptake of sitosterol by the BBM is energy-independent and facilitated in a manner analogous to cholesterol uptake [Thurnhofer, H., & Hauser, H. (1990a) Biochemistry 29, 2142-2148]. (V) The rate of cholesterol and sitosterol uptake by BBMV from both mixed bile salt micelles and small unilamellar vesicles (SUV) as the donor is directly proportional to the sterol content of the donor. (VI) The pseudo-first-order rate constants k1 for sterol uptake from SUV are independent of the sterol content up to 10-20 mol %. Above that, competition between the two sterols leads to a reduction of the k1 values.
References
Oct 1, 1985·Analytical Biochemistry·P K SmithD C Klenk
Apr 1, 1981·The American Journal of Clinical Nutrition·G V VahounyL L Gallo
Jan 1, 1980·Journal of Nutritional Science and Vitaminology·Y ShidojiN Hosoya
Jun 1, 1995·Biophysical Journal·G J SömjenH Hauser
May 1, 1995·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·E LevyE Seidman
Feb 1, 1993·The Journal of Pediatrics·M BeckerK Von Bergmann
Citations
Mar 30, 2002·Biochimica Et Biophysica Acta·Scott W AltmannMichael P Graziano
Sep 11, 1999·Current Opinion in Lipidology·P A Dawson, L L Rudel
Jul 16, 2003·Cold Spring Harbor Symposia on Quantitative Biology·H H HobbsJ C Cohen
Jun 13, 2008·Lipids in Health and Disease·Sheila J ThorntonKishor M Wasan
Sep 11, 2003·Clinical Chemistry and Laboratory Medicine : CCLM·Patrick Borel
Aug 15, 2009·Atherosclerosis·Don S LinWilliam E Connor
Jul 7, 1997·FEBS Letters·D BoffelliH Hauser
Apr 20, 2007·Biochimica Et Biophysica Acta·Eric D LabontéDavid Y Hui
Nov 29, 2011·Analytical Biochemistry·Nanako ItoHideki Hakamata
Oct 7, 2005·Atherosclerosis·H K GyllingT A Miettinen
Apr 19, 2002·Nutrition and Cancer·A B AwadC S Fink
Sep 16, 2005·Journal of Lipid Research·Lily JakuljMaud N Vissers
Feb 6, 2019·Nutrients·Takanari NakanoTakayuki Murakoshi
Jul 31, 2004·The Journal of Nutrition·Jiang HuPatricia A Murphy
Oct 21, 2004·The Journal of Biological Chemistry·Werner KramerGerd Schmitz
Feb 23, 2021·Critical Reviews in Food Science and Nutrition·Simin FengChung S Yang
Sep 16, 2005·Journal of Medicinal Chemistry·Lisbet KvaernøErick M Carreira
Sep 19, 2001·Biochemistry·M WerderH Hauser