Comparison of Colilert-18 with miniaturised most probable number method for monitoring of Escherichia coli in bathing water

Journal of Water and Health
Ananda TiwariTarja Pitkänen

Abstract

The purpose of this equivalence study was to compare an alternative method, Colilert-18 Quanti-Tray (ISO 9308-2) with the European bathing water directive (2006/7/EC) reference method, the miniaturised most probable number (MMPN) method (ISO 9308-3), for the analysis of Escherichia coli. Six laboratories analysed a total of 263 bathing water samples in Finland. The comparison was carried out according to ISO 17994:2004. The recovery of E. coli using the Colilert-18 method was 7.0% and 8.6% lower than that of the MMPN method after 48 hours and 72 hours of incubation, respectively. The confirmation rate of presumptive E. coli-positive wells in the Colilert-18 and MMPN methods was high (97.8% and 98.0%, respectively). However, the testing of presumptive E. coli-negative but coliform bacteria-positive (yellow but not fluorescent) Colilert-18 wells revealed 7.3% false negative results. There were more false negatives in the naturally contaminated waters than in the samples spiked with waste water. The difference between the recovery of Colilert-18 and the MMPN method was considered not significant, and subsequently the methods are considered as equivalent for bathing water quality monitoring in Finland. Future bathing water method e...Continue Reading

References

Jan 5, 2002·Journal of Microbiological Methods·Annie RompréPatrick Laurent
Feb 2, 2002·Applied and Environmental Microbiology·John M PisciottaValerie J Harwood
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Sep 8, 2009·Marine Pollution Bulletin·Catarina R MansilhaPaula Gameiro
Feb 23, 2013·Journal of Water and Health·Anna-Maria HokajärviIlkka T Miettinen

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Citations

Oct 25, 2016·Marine Pollution Bulletin·Darija Vukić LušićDražen Lušić
Jun 3, 2021·International Journal of Environmental Research and Public Health·Ananda TiwariTarja Pitkänen
Jun 5, 2021·Applied and Environmental Microbiology·Ana Carolina de Mello SantosTânia Aparecida Tardelli Gomes

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