PMID: 9542937May 23, 1998Paper

Comparison of culture- and non-culture-based methods for quantification of viral load and resistance to antiretroviral drugs in patients given zidovudine monotherapy

Journal of Clinical Microbiology
Richard S TedderJ H Darbyshire

Abstract

Virological assays for human immunodeficiency virus type 1 load and drug resistance can broadly be divided into culture-based and molecular biology-based methods. Culture-based methods give a direct measure of infectious virus load and phenotypic drug resistance, whereas molecular biology-based methods are indirect, assaying nucleic acid levels to determine virus load and point mutations associated with drug resistance. We have compared culture-based and non-culture-based methods for patients enrolled in a placebo-controlled trial of zidovudine (the Concorde Trial). Virus loads were assayed by culture of peripheral blood mononuclear cells (PBMCs) or quantitative PCR, and drug resistance was assayed in culture or in a quantitative, PCR-based point mutation assay. The rates of detection of viremia and drug resistance were higher by PCR than by culture for this population of subjects. Comparison of the virus loads by the two measures showed a good correlation for virus loads in PBMCs but a poor correlation for virus loads in plasma. The latter result probably reflected the inaccuracies of culture in assaying plasma with the low infectious virus titers seen in the study population. The concordance of phenotypic and genotypic drug r...Continue Reading

References

Mar 1, 1992·Proceedings of the National Academy of Sciences of the United States of America·P KellamB A Larder
Jul 1, 1992·AIDS Research and Human Retroviruses·S Aoki-SeiH Mitsuya
Dec 14, 1989·The New England Journal of Medicine·R W CoombsL Corey
Dec 14, 1989·The New England Journal of Medicine·D D HoM Alam
Feb 1, 1993·Journal of Virological Methods·M G SempleR S Tedder

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Citations

Aug 17, 1999·Sexually Transmitted Infections·Q Abdool Karim, S S Abdool Karim
Jun 22, 2000·Clinical Infectious Diseases : an Official Publication of the Infectious Diseases Society of America·P R Harrigan, H C Côté

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