PMID: 6979558May 1, 1982Paper

Comparison of culture and microscopy in the diagnosis of Gardnerella vaginalis infection

Journal of Clinical Pathology
C A IsonC S Easmon

Abstract

A comparison was made between human blood agar containing amphotericin B, nalidixic acid and either gentamicin or colistin for the isolation of Gardnerella vaginalis from cases of non-specific vaginitis seen in a clinic for sexually transmitted diseases. The medium containing gentamicin was more inhibitory for non-Gardnerella species, but not sufficiently inhibitory to allow direct plating in the clinic without spreading for single colonies. The diffuse beta haemolysis produced by G vaginalis on human, but not on horse blood agar, proved very useful in differentiating it from other vaginal organisms and was not affected by the antibiotics used. This characteristic, together with Gram stain morphology, oxidase and catalase, provides a simple, reliable methods of identifying G vaginalis. Sixty women with symptoms of vaginitis, in whom no other pathogen was isolated, were examined by culture and microscopy. Gardnerella vaginalis was grown from 45 whereas only 31 had positive microscopy (clue cells or Gram-variable bacilli). There was no significant difference between the rate of isolation of G vaginalis in the group with positive microscopy (25/31) and that with negative microscopy (20/31).

References

Dec 1, 1977·The Journal of Infectious Diseases·W M McCormackS H Zinner
Jun 29, 1978·The New England Journal of Medicine·T A PheiferK K Holmes
Feb 1, 1979·Journal of Clinical Microbiology·J R Greenwood, M J Pickett
Aug 1, 1979·Antimicrobial Agents and Chemotherapy·L R McCarthyE G Smith
Jun 1, 1979·Journal of Clinical Microbiology·R F Smith
Jan 1, 1979·Journal of Clinical Microbiology·R K BaileyR F Smith
Jan 15, 1979·American Journal of Obstetrics and Gynecology·M E LevisonC N Floro
Jan 1, 1977·American Journal of Obstetrics and Gynecology·M E LevisonD Kaye
Sep 11, 1980·The New England Journal of Medicine·C A SpiegelK K Holmes

❮ Previous
Next ❯

Citations

Apr 1, 1986·European Journal of Clinical Microbiology·M N Chowdhury, S K Desilva
Oct 1, 1982·European Journal of Clinical Microbiology·D MilatovicI Braveny
Oct 1, 1982·European Journal of Clinical Microbiology·J C Clay
Sep 1, 1984·The Journal of Infection·B Chattopadhyay
Jul 1, 1988·Zentralblatt Für Bakteriologie, Mikrobiologie, Und Hygiene. Series A, Medical Microbiology, Infectious Diseases, Virology, Parasitology·P ElsnerI Wecker
Dec 1, 1983·Journal of Clinical Pathology·C A IsonJ W Harris
Apr 1, 1985·Genitourinary Medicine·C A Ison, C S Easmon
Jun 1, 1987·Genitourinary Medicine·C A IsonC S Easmon
Jun 1, 1990·Genitourinary Medicine·P R MasonE Marowa
Apr 17, 2007·Best Practice & Research. Clinical Obstetrics & Gynaecology·Gilbert G G Donders
Mar 1, 1984·The Journal of Infection·B Chattopadhyay, J C Teli
Aug 1, 1982·The British Journal of Venereal Diseases·S G DawsonC S Easmon
Dec 1, 1992·British Journal of Obstetrics and Gynaecology·C M StaceyR W Beard
Dec 1, 1994·British Journal of Obstetrics and Gynaecology·P E HayD Taylor-Robinson
Feb 1, 1983·Postgraduate Medicine·G T Schneider
Sep 1, 1985·Scandinavian Journal of Primary Health Care·H Schmidt, J G Hansen
Jul 1, 1983·Journal of Clinical Microbiology·C A SpiegelK K Holmes
Aug 1, 1983·Journal of Clinical Microbiology·S Ratnam, B L Fitzgerald
Oct 1, 1991·Clinical Microbiology Reviews·C A Spiegel
Sep 1, 1989·Journal of Clinical Microbiology·M GhioneE Magliano

❮ Previous
Next ❯

Related Concepts

Related Feeds

Antifungals

An antifungal, also known as an antimycotic medication, is a pharmaceutical fungicide or fungistatic used to treat and prevent mycosis such as athlete's foot, ringworm, candidiasis, cryptococcal meningitis, and others. Discover the latest research on antifungals here.

Antifungals (ASM)

An antifungal, also known as an antimycotic medication, is a pharmaceutical fungicide or fungistatic used to treat and prevent mycosis such as athlete's foot, ringworm, candidiasis, cryptococcal meningitis, and others. Discover the latest research on antifungals here.