Comparison of DATs using traditional tube agglutination to gel column and affinity column procedures

Transfusion
K DittmarD F Stroncek

Abstract

Detection of immunoglobulin or complement bound to RBCs by using the DAT is valuable in the diagnosis of immune-mediated hemolytic anemia. Traditionally, the DAT has been performed by tube agglutination using anti-IgG or anti-C3d. The purpose of this study was to compare the tube agglutination DAT to gel microcolumn, affinity microcolumn, and flow cytometric DATs on RBCs coated in vitro and on patient RBC samples. RBCs from 84 patients were assessed by tube agglutination DAT, one gel microcolumn DAT, and two affinity microcolumn DATs. One affinity microcolumn assay was unmodified and one was modified by the addition of polyspecific antiglobulin or anti-IgG as a secondary antibody. RBCs from 15 of the 84 patients underwent analysis by flow cytometry with fluorescence-labeled anti-IgG. The assays were also compared by using D+ RBCs sensitized with serially adjusted concentrations of anti-D. Both tube agglutination and gel microcolumn DATs were positive in 49 patient samples; both assays were negative in 20 samples, and the results were discordant in 15. Gel microcolumn DATs were more likely than were tube agglutination DATs to detect IgG on RBCs. Affinity microcolumn DATs were less likely than gel microcolumn or tube agglutinatio...Continue Reading

References

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Citations

Sep 20, 2005·Journal of Clinical Laboratory Analysis·M C Z NovarettiD A F Chamone
Jan 15, 2014·Blood Cells, Molecules & Diseases·George B Segel, Marshall A Lichtman
Jul 31, 2002·Transfusion·Victoria A GrecoDavid F Stroncek
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Sep 10, 2004·Journal of Clinical Laboratory Analysis·M C Z NovarettiD A F Chamone
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Mar 31, 2015·Transfusion and Apheresis Science : Official Journal of the World Apheresis Association : Official Journal of the European Society for Haemapheresis·Jamil M A S ObaidBasma H M Mersal
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Dec 6, 2016·Transfusion·Alyssa ZimanUNKNOWN the Biomedical Excellence for Safer Transfusion (BEST) Collaborative
Jun 11, 2021·Transfusion clinique et biologique : journal de la Société française de transfusion sanguine·J CaboV Deneys

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