Comparison of detection techniques for capillary electrophoresis analysis of gold nanoparticles

Electrophoresis
Magdalena MatczukAndrei R Timerbaev

Abstract

As metallic nanoparticles are growing in importance as analytes in CE, increases an interest in appropriate detection methods for their quantification in various samples. For gold nanoparticles (AuNPs), the most common UV detection poses intricacy of inadequate sensitivity that hinders the applicability of CE. With the objective of resolving this challenge, UV detection was compared with C(4) D and ICP-MS as alternative modes of detection for AuNPs. A C(4) D detector, applied under pressure-driven conditions, exhibited better sensitivity than a UV detector. However, C(4) D turned to be unsatisfactory to differentiate the signal of AuNPs at common CE conditions despite varying the nature of BGE and detection conditions. Due to intrinsic sensitivity and low background levels typical to Au, ICP-MS greatly surpasses UV detection. After optimization trials, CE-ICP-MS gained the LOD of AuNPs as low as 2 × 10(-15) M, as well as an excellent performance in terms of signal stability and linearity. Also importantly, the optimized BGE appears to be well matched to explore the behavior of AuNPs in biologically relevant systems. This was demonstrated by probing the interaction between AuNPs and the main blood-transporting protein, HSA.

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Citations

Jun 9, 2016·Electrophoresis·Andrea Mathilde MebertMartín Federico Desimone
Oct 27, 2016·Electrophoresis·Anna TýčováKarel Klepárník
Jul 1, 2017·Electrophoresis·Vojtech Adam, Marketa Vaculovicova
Jun 9, 2018·Electrophoresis·Tereza VaneckovaMarketa Vaculovicova
Nov 30, 2018·Nanomaterials·Pengfei JiangLibo Nie
Jan 12, 2021·Critical Reviews in Analytical Chemistry·Carlos AdelantadoÁngel Ríos
Feb 14, 2021·Analytical and Bioanalytical Chemistry·Freja Grønbæk-ThorsenLaura Hyrup Møller

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