PMID: 6171860Jun 1, 1981Paper

Comparison of different analytical and precipitation methods for direct estimation of serum high-density lipoprotein cholesterol

Scandinavian Journal of Clinical and Laboratory Investigation
I PenttilãP Juutilainen

Abstract

In this study two commercial enzymatic methods for the determination of serum cholesterol have been adapted for a continuous-flow analyser and a discrete multichannel analyser. The selective precipitation methods for lipoproteins using either dextran sulphate/MgCl2, heparin/MnCl2, phosphotungstate/MgCl2 or polyethylene glycol (mol. wt 6000) were compared with each other and with ultracentrifugal analysis as well as with agarose gel electrophoresis for lipoproteins. The results indicated that the dextran sulphate/MgCl2 precipitation procedure was the method of choice for the determination of HDL-cholesterol from clear and hyperlipemic sera. HDL-cholesterol values for 391 apparently health women (X +/- SD) were 1.58 +/- 0.34 mmol/l and for 432 men 1.27 +/- 0.29 mmol/L. The corresponding HDL-cholesterol/cholesterol ratios for women were 0.292 +/- 0.068 and for men 0.229 +/- 0.069. The HDL-cholesterol in serum was slightly age dependent in women, while the HDL-cholesterol/cholesterol ratio showed highly significant negative correlation with the age in both women and men.

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Citations

Dec 1, 1987·Clinica Chimica Acta; International Journal of Clinical Chemistry·I M PenttiläE Voutilainen
Jan 1, 1994·Atherosclerosis·E S SarkkinenY A Kesäniemi
Dec 1, 1983·European Journal of Obstetrics, Gynecology, and Reproductive Biology·I M PenttiläO Castrén
Sep 6, 2000·Thrombosis Research·T RankinenR Rauramaa
May 27, 2003·Molecular Genetics and Metabolism·Virpi LindiUNKNOWN KANWU Study Group
Jul 13, 1995·The New England Journal of Medicine·J PartanenM Uusitupa
Mar 1, 2007·Nutrition, Metabolism, and Cardiovascular Diseases : NMCD·Virpi LindiUNKNOWN KANWU Study Group
Mar 14, 2000·Redox Report : Communications in Free Radical Research·D E LaaksonenC K Sen
Jul 27, 1999·Metabolism: Clinical and Experimental·A M LouherantaM I Uusitupa
Feb 1, 1994·Scandinavian Journal of Clinical and Laboratory Investigation·J MaatelaM J Tikkanen
Apr 12, 2001·European Journal of Clinical Investigation·J PihlajamäkiM Laakso
Jul 1, 1992·Arteriosclerosis and Thrombosis : a Journal of Vascular Biology·M I UusitupaK Pyörälä
Oct 1, 1993·Arteriosclerosis and Thrombosis : a Journal of Vascular Biology·P KarhapääM Laakso

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