PMID: 6169739Oct 1, 1981Paper

Comparison of different antinucleic acid antibody spectrotypes in spontaneous, induced, and murine lupus

The Journal of Clinical Investigation
M FischbachN Talal

Abstract

Sera from patients with systemic lupus erythematosus and from mice spontaneously developing lupus were subjected to isoelectric focusing by a microsucrose gradient method. The spectrotypes of human antibodies to native DNA, denatured DNA, and polyriboadenylic acid (poly A) were compared. Antibodies to native DNA and denatured DNA focused into two regions whose boundaries were pH 5.0-7.0 and pH 8.5-10.0. Antinative DNA antibodies were more homogeneous than antidenatured DNA antibodies. Anti-DNA antibodies in cryoglobulins showed more restriction than those present in serum. There was no relationship between spectrotype and pattern of disease expression. Murine antibodies to native DNA were more heterogeneous than human anti-DNA antibodies. The spectrotypes of antidenatured DNA antibodies from patients with systemic lupus erythematosus or drug induced lupus, or from an immunized rabbit, were similar. Likewise, antibodies to poly A were similar in both human and murine lupus. In contrast to anti-DNA, antibodies to poly A were restricted and focused only in the alkaline range (pH 9.5-10.0). The spectrotype of antipoly A antibodies induced by lipopolysaccharide were comparable but had an additional small band at pH 6.2. Our results ...Continue Reading

References

Oct 1, 1967·The Journal of Experimental Medicine·D KofflerH G Kunkel
Sep 1, 1973·Immunochemistry·J L Press, N R Klinman
Jul 1, 1974·Analytical Biochemistry·S C MarchP Cuatrecasas
Jul 1, 1971·The Journal of Experimental Medicine·D KofflerH G Kunkel
Aug 1, 1964·Proceedings of the National Academy of Sciences of the United States of America·O J PLESCIAN C PALCZUK

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Citations

Aug 1, 1985·The Journal of Clinical Investigation·H YoshidaP H Lambert
Dec 1, 1984·Arthritis and Rheumatism·J E PersselinR H Stevens
Dec 1, 1993·Clinical and Experimental Immunology·R Suenaga, N I Abdou

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