PMID: 7521978Jun 1, 1994Paper

Comparison of different assays for the quantitation of FK 506 levels in blood or plasma

Therapeutic Drug Monitoring
M WinklerR Pichlmayr

Abstract

In a prospective study, we evaluated the analytical performance of different assays for the quantitation of FK 506 in blood and plasma. The new whole blood IMX assay was compared with four modifications (liquid/liquid or solid/liquid extraction of blood or plasma respectively) of the standard enzyme-linked immunosorbent assay (ELISA). Both assays utilize the same monoclonal antibody for detection of FK 506. Using isolated FK 506 metabolites generated in vitro, this antibody was shown to recognize not only the unmodified parent drug but also FK 506 metabolites. In stable liver-grafted patients, sensitivity of both modifications of the whole blood ELISA was adequate. In contrast, in some stable patients, plasma ELISA was not able to detect FK 506; this was observed mainly after liquid/liquid extraction of FK 506. Sensitivity of the IMX assay was also low; in six of 10 stable liver-grafted patients, IMX levels were below the limit of detection. In all assays, FK 506 levels were found to be elevated during episodes of liver dysfunction; a possible explanation for this effect would be that of accumulation of cross-reacting FK 506 metabolites. As FK 506 metabolites are known to be less immunosuppressive than the parent drug, liver fu...Continue Reading

Citations

Aug 1, 1994·Transplant International : Official Journal of the European Society for Organ Transplantation·M WinklerR Pichlmayr
Dec 29, 1998·Transplantation Proceedings·F OdoulM Breteau
Jan 1, 1994·Transplant International : Official Journal of the European Society for Organ Transplantation·M WinklerC Groth
May 22, 2001·Journal of Clinical Pharmacology·R VenkataramananA Shaked
Aug 1, 1996·Therapeutic Drug Monitoring·D W HoltA Johnston
Apr 21, 1998·Annals of Clinical Biochemistry·A K Trull

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