PMID: 7035616Jan 1, 1981Paper

Comparison of different methods for the detection of rubella-specific IgM antibodies

Journal of Medical Virology
R BraunK Munk

Abstract

The rubella specific IgM titer in the serum specimens originating from healthy persons and from patients with clinical signs of rubella infection was determined by hemagglutination inhibition or hemagglutination reduction after IgM separation with the following methods: (a) density gradient centrifugation; (b) polyacrylamide agarose gel chromatography; (c) ion exchange chromatography with diethylaminoethyl cellulose columns; (d) solid-phase immunosorbent technique using microplates; (e) solid-phase immunosorbent technique using polyacrylamide microimmunobeads. Alternatively, we removed IgG and IgA by the use of protein A, anti-IgG, and anti-IgA, covalently coupled to controlled-pore glass (f). The titers obtained by the different methods showed qualitatively good correlations when combined with mercaptoethanol reduction. The quantitative measurement of specific IgM titers, however, revealed a lower sensitivity of column chromatography and methods of removal of IgG/IgA.

References

Apr 1, 1976·Journal of Endodontics·S GulmenL W O'Brien
Feb 1, 1976·The Journal of Hygiene·J E Cradock-Watson, M K Ridehalgh
Feb 1, 1975·Journal of Clinical Microbiology·H SchmitzH W Doerr

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Citations

Jan 1, 1986·Archives of Virology·J V Parry, S D Gardner
Jan 1, 1982·Archives of Virology·A CoralliniG Barbanti-Brodano
Mar 17, 2004·Journal of Clinical Virology : the Official Publication of the Pan American Society for Clinical Virology·A Behzad-BehbahaniS H Khoo
Jan 1, 1983·Medical Microbiology and Immunology·T MertensH J Eggers
Sep 1, 1995·European Journal of Clinical Microbiology & Infectious Diseases : Official Publication of the European Society of Clinical Microbiology·V LuyasuJ Macioszek
Jun 1, 1988·Molecular and Cellular Probes·R H Yolken
Feb 1, 1985·Journal of Clinical Pathology·H Cubie, E Edmond
May 1, 1984·Journal of Clinical Pathology·J Hodgson, P Morgan-Capner
Aug 1, 1991·Epidemiology and Infection·J E Cradock-Watson
Sep 3, 1984·Klinische Wochenschrift·K GmelinB Kommerell
Mar 1, 1990·Journal of Clinical Microbiology·P ShankaranE P Diamandis

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