Comparison of different microscopy approaches to quantification of inhibitory effect on thrombus formation under flow conditions by the example of adenosine receptor agonist HE-NECA

Journal of Pharmacological and Toxicological Methods
Tomasz PrzygodzkiCezary Watala

Abstract

Thrombus formation in vitro in flow conditions and its visualization and quantification with the use of microscopy are widely utilized to evaluate activity of compounds with a potential antithrombotic activity. Visualization and quantification of thrombi can be performed with the use of wide-field or confocal microscopy. Acquiring reliable numerical data from wide-field microscopy images of objects which have a complex three-dimensional structure is strongly influenced by the methods used for image analysis. This can be a possible source of inaccuracy in assessment of antithrombotic activity of a tested substance. We aimed to verify how different approaches to the quantification of wide-field images can affect the evaluation of an antiplatelet effect of a tested substance. We compared three algorithms of image analysis to evaluate an effect of 2-hexynyl-5'-ethylcarboxamidoadenosine (HE-NECA), a compound of a moderate antiplatelet activity on thrombus formation, and of abciximab - a potent antiplatelet compound. Also, we studied how the results obtained in a wide-field imaging correspond to those obtained by means of confocal imaging. Three algorithms for analysis of wide-field images showed antiplatelet effect of HE-NECA or abc...Continue Reading

Citations

Nov 7, 2019·International Journal of Molecular Sciences·Nina Wolska, Marcin Rozalski
Jan 8, 2020·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Nina WolskaMarcin Rozalski

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