Comparison of different PCR approaches for characterization of Burkholderia (Pseudomonas) cepacia isolates.

Journal of Clinical Microbiology
P Y LiuC Y Tseng

Abstract

In this study, we evaluated three PCR methods for epidemiological typing of Burkholderia (Pseudomonas) cepacia--PCR-ribotyping, arbitrarily primed PCR (AP-PCR) and enterobacterial repetitive intergenic consensus sequence PCR (ERIC-PCR)--and compared them with pulsed-field gel electrophoresis. The analysis was performed with 31 isolates of B. cepacia, comprising 23 epidemiologically unrelated isolates and 8 isolates collected from the same patient during two episodes of bacteremia. Pulsed-field gel electrophoresis, ERIC-PCR, and AP-PCR identified 23 distinct types among the 23 unrelated isolates, while PCR-ribotyping only identified 12 strain types, even after AluI digestion of the amplification products. Among the eight isolates collected from the same patient, all typing techniques revealed two clones of strains. The day-to-day reproducibilities of PCR-ribotyping and ERIC-PCR were good, while greater day-to-day variations were noted in the fingerprints obtained by AP-PCR. We conclude that all three PCR techniques are useful for rapid epidemiological typing of B. cepacia, but ERIC-PCR seems to be more reproducible and discriminative.

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Citations

Sep 18, 2002·Journal of Applied Microbiology·J RichardsonR H A Coutts
Feb 6, 2019·Annals of Clinical Microbiology and Antimicrobials·Naveen Kumar Devanga Ragupathi, Balaji Veeraraghavan
Sep 28, 2001·Journal of Clinical Microbiology·T CoenyeJ J LiPuma
Oct 7, 2005·Journal of Clinical Microbiology·Preeti PancholiPhyllis Della Latta
Aug 1, 1997·Journal of Clinical Microbiology·C SegondsG Chabanon
Mar 4, 2000·Journal of Clinical Microbiology·V A de la Puente-RedondoE F Rodríguez Ferri
Nov 29, 2001·Journal of Clinical Microbiology·H C Wong, C H Lin

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