Comparison of different polar compounds-induced cytotoxicity in human hepatocellular carcinoma HepG2 cells

Lipids in Health and Disease
Jinwei LiYuanfa Liu

Abstract

Total polar compounds (TPC) formed during successive frying have the negative healthy effects. However, little researches focused on the cytotoxicity of different parts of TPC. The present study was carried out to elucidate the different polar compounds-induced apoptosis in human hepatocellular carcinoma (HCC) HepG2 cells. The polar compounds of frying oil named oxidized triglycerides oligo (TGO), oxidized triglycerides dimer (TGD), and oxidize triglycerides (ox-TG) were isolated and collected via HPLC. MTT assay was selected to determine the cell viability, and apoptosis rate of the cells was analyzed with the help of flow cytometry. The results indicated that TGO, TGD, or ox-TG could suppress the proliferation of HepG2 cells and improve the cell apoptosis in the concentration- and time- dependent manner. Different polar compounds have the different activity of cytotoxicity and apoptosis (p < 0.05), and ox-TG was the most serious deleterious on HepG2 cell viability and apoptotic, followed by TGO and TGD. At the concentration of 2.00 mg/ml and incubated for 48 h, the cell inhibition rate and apoptotic rate of HepG2 induced by ox-TG could reach 23.0 % and 16.05 %, respectively. Cell cycle analysis showed that the inhibition of T...Continue Reading

References

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Citations

Jun 12, 2020·Journal of Clinical Pharmacy and Therapeutics·Zohereh OghabianSomayyeh Karami-Mohajeri
Feb 8, 2019·Journal of Food Science and Technology·Carla S P SantosSusana Casal
Jun 28, 2019·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Azmil Haizam Ahmad TarmiziAinie Kuntom
Jan 26, 2021·Journal of Lipids·Hailemariam AmsaluGesese Bogale
Nov 18, 2021·Journal of the Science of Food and Agriculture·Xue LiYong-Jiang Xu
Dec 23, 2021·Molecular Omics·Jiachen ShiYong-Jiang Xu
Dec 24, 2021·Journal of Evidence-based Integrative Medicine·Nateelak KooltheatMoragot Chatatikun
Nov 26, 2020·Food Research International·Lirong XuXingguo Wang

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Methods Mentioned

BETA
flow cytometry

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