Comparison of different tandem mass spectrometric techniques (ESI-IT, ESI- and IP-MALDI-QRTOF and vMALDI-TOF/RTOF) for the analysis of crocins and picrocrocin from the stigmas of Crocus sativus L

Rapid Communications in Mass Spectrometry : RCM
Nikolaos Stavros KoulakiotisGünter Allmaier

Abstract

The expensive spice saffron originating from the stigmas of Crocus sativus L. and also applied in traditional Chinese medicine (TCM) constitutes a complex mixture of glycoconjugates varying not only in the aglycon structure, but also in glycosylation pattern. Therefore, various tandem mass spectrometric techniques were evaluated for their usefulness in structural elucidation. Three selected constituents of the stigmas of Crocus sativus L., trans- and cis-crocin-4 as well as picrocrocin, were isolated and purified by HPLC and finally analyzed by ESI-MS (ion trap, QqRTOF), IP-MALDI-MS (QqRTOF) and vMALDI-MS (TOF/RTOF) in combination with tandem mass spectrometry in collision energy regimes ranging from a few eV (LE) to 20 keV (HE) collisions for the first time. These data aid in structurally elucidating minor, unknown glycoconjugates originating from this plant-derived spice. LE-CID of isomeric crocins on either an ion trap with ESI or a QqRTOF-instrument with ESI or IP-MALDI as desorption/ionization technique only yielded a limited number of structurally diagnostic sodiated product ions related to the carbohydrate moiety as well as to the intact aglycon in contrast to true HE-CID. The low MW constituent picrocrocin did not yield...Continue Reading

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Citations

Sep 24, 2013·Food Chemistry·Lina CossignaniFrancesca Blasi
Mar 24, 2016·Proteomics·Ernst PittenauerGünter Allmaier
Nov 7, 2014·Plant Foods for Human Nutrition·Lidia Rodríguez-NeiraJulia López-Hernández
Jul 26, 2018·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Angelo Antonio D'ArchivioFabrizio Ruggieri
Oct 25, 2020·Scientific Reports·Nikolaos Stavros KoulakiotisAnthony Tsarbopoulos
Dec 3, 2020·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Maria Anna MaggiCristiana Picco
May 1, 2021·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Evangelos GikasAnthony Tsarbopoulos

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