Comparison of different vitrification protocols on viability after transfer of ovine blastocysts in vitro produced and in vivo derived

Theriogenology
M DattenaP Cappai

Abstract

We compare different vitrification protocols on the pregnancy and lambing rate of in vitro produced (IVP) and in vivo derived (IVD) ovine embryos. Ovine blastocysts were produced by in vitro maturation, fertilization and culture of oocytes collected from slaughtered ewes or superovulated and inseminated animals. Embryos were cryopreserved after exposure at room temperature either for 5 min in 10% glycerol (G), then for 5 min in 10% G + 20% ethylene glycol (EG), then for 30 s in 25% G + 25% EG (glycerol group), or for 3 min in 10% EG + 10% dimethyl sulphoxide (DMSO), then for 30s in 20% EG + 20% DMSO + 0.3 M sucrose (DMSO group). One group of in vitro produced embryos was cryopreserved similarly to the DMSO group, but with 0.75 M sucrose added to the vitrification solution (DMSO 0.75 group). Glycerol group embryos were then loaded into French straws or open pulled Straws (OPS) while the DMSO group embryos were all loaded into OPS and directly plunged into liquid nitrogen. Embryos were warmed with either a one step or three step process. In the one step process, embryos were placed in 0.5 M sucrose. The three-step process was a serial dilution in 0.5, 0.25 and 0.125 M sucrose. The embryos of DMSO 0.75 group were warmed directly b...Continue Reading

References

Jul 1, 1976·New Zealand Veterinary Journal·H R Tervit, P G Havik
Jan 1, 1990·Reproduction, Fertility, and Development·A SzéllR Hudson
Mar 1, 1989·Biology of Reproduction·P B Seshagiri, B D Bavister
Nov 1, 1986·Journal of Reproduction and Fertility·A Széll, J N Shelton
Jan 1, 1986·Journal of Reproduction and Fertility·A Széll, J N Shelton
Sep 1, 1972·Journal of Reproduction and Fertility·H R TervitL E Rowson
Jan 1, 1995·Reproduction, Nutrition, Development·A MassipF Dessy
May 1, 1994·Zentralblatt für Veterinärmedizin. Reihe A·M Shamsuddin, H Rodriguez-Martinez
Sep 1, 1993·Journal of Reproduction and Fertility·J Ali, J N Shelton
May 1, 1993·Journal of Reproduction and Fertility·S E ZhuT Machida
Jul 1, 1993·Journal of Reproduction and Fertility·J Ali, J N Shelton
Aug 26, 1998·Molecular Reproduction and Development·G VajtaH Callesen
Mar 25, 2000·Theriogenology·A G Martínez, M Matkovic
May 24, 2003·Theriogenology·Vladimir IsachenkoJosé Luis Alabart
Aug 1, 1979·Theriogenology·A MassipC Hanzen
Oct 1, 1990·Theriogenology·E Yuswiati, W Holtz
Mar 1, 1996·Theriogenology·J S Vicente, F García-Ximénez

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Citations

Dec 25, 2002·Theriogenology·Y CogniéP Mermillod
Mar 8, 2013·Archives of Gynecology and Obstetrics·Farideh YazdanpanahHojatallah Karimi
Mar 13, 2014·Theriogenology·Sara SuccuFiammetta Berlinguer
Oct 29, 2008·Reproduction in Domestic Animals = Zuchthygiene·R E GreenS D Bicudo
Aug 25, 2006·Molecular Reproduction and Development·M DattenaP Cappai
Jun 11, 2008·Molecular Reproduction and Development·Florence GuignotPascal Mermillod
Dec 3, 2010·Reproduction in Domestic Animals = Zuchthygiene·F GuignotG Baril
May 30, 2014·Reproduction, Fertility, and Development·M H BhatR A Shah
Sep 5, 2013·Zygote : the Biology of Gametes and Early Embryos·L MaraI M Mayorga Muñoz
Aug 14, 2019·Reproduction in Domestic Animals = Zuchthygiene·Lucas Machado FigueiraJoanna Maria Gonçalves Souza-Fabjan
Nov 23, 2019·Journal of Animal Science and Biotechnology·Sergio LeddaAmir Arav

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