PMID: 3041953May 1, 1988Paper

Comparison of direct electron microscopy and enzyme immunoassay for the detection of rotaviruses in calves, lambs, piglets and foals

Australian Veterinary Journal
G R Ellis, E Daniels

Abstract

Direct electron microscopy (EM) and enzyme-immunoassay (rotazyme) results for the detection of rotaviruses in 346 enteric specimens from calves, lambs, piglets and foals were compared. The rotazyme test was at least 3 times more sensitive than direct EM in diagnosing infection. Rotavirus antigen was demonstrated by rotazyme in 22% of 280 scour samples and in 27% of 66 samples from non-scouring animals. There was an association between diarrhoea and higher amounts of rotavirus antigen. This prevalence of rotaviruses detected in animals with diarrhoea highlights the significant involvement of other pathogens identified in the study including Eimeria, Cryptosporidia, Escherichia coli, Campylobacter, and other viruses.

References

Feb 1, 1985·Applied and Environmental Microbiology·R D Pontefract, G Bergeron
Aug 1, 1985·Australian Veterinary Journal·P J O'Donoghue
Jul 1, 1980·Journal of Clinical Microbiology·A NicolaieffM H van Regenmortel
May 1, 1982·Journal of Clinical Microbiology·A S Rubenstein, M F Miller
Feb 1, 1984·Australian Veterinary Journal·J A MarshallI D Gust

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Citations

Jul 16, 2008·Veterinary Research Communications·K DhamaS V S Malik
Dec 1, 1989·Australian Veterinary Journal·I W Caple
Jan 9, 2003·Journal of Clinical Microbiology·Roger K MaesRobert Holland
Aug 1, 1989·Australian Veterinary Journal·R S Campbell
Oct 1, 1989·Alcoholism, Clinical and Experimental Research·T J McCown, G R Breese
Apr 1, 2004·Small Ruminant Research : the Journal of the International Goat Association·S A WaniA S Buchh

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