Comparison of DNA adduct induction in vitro by PAHs and nitro-pahs in freshly isolated rat hepatocytes

Toxicology in Vitro : an International Journal Published in Association with BIBRA
S X Qu, N H Stacey

Abstract

Polycyclic aromatic hydrocarbons (PAHs) and nitro-PAHs have been identified widely in occupational and environmental pollution, such as diesel engine emissions and other combustion products. In most cases, hepatic biotransformation is involved in converting these chemicals to their carcinogenic metabolites. It has been demonstrated that isolated hepatocytes possess substantial amounts of the enzymes responsible for metabolizing xenobiotics and are therefore a convenient model for studying chemicals that require activation to exert their carcinogenic effects. In this study, rat hepatocytes were isolated by collagenase digestion and then exposed to benzo[a]pyrene (B) [a]P), benzo[a]anthracene (B[a]A), 1-nitropyrene (1-NP) and 1,6-dinitropyrene (1,6-DNP) at different doses and/or times so that DNA adducts levels, as measured with the (32)P-postlabelling technique, could be compared. Each of the four compounds tested induced significant increases of total DNA adducts with clear dose-related responses. One or more individual adducts were identified as major adducts for each compound. Time-related increases of DNA adducts were also observed from 1 to 4 hr of incubation. Greater amounts of DNA adducts were induced by B[a]P or 1,6-DNP ...Continue Reading

References

Jul 1, 1994·Food and Chemical Toxicology : an International Journal Published for the British Industrial Biological Research Association·C W SheuP P Fu
Apr 1, 1994·Toxicology in Vitro : an International Journal Published in Association with BIBRA·R W West, K L Rowland
May 1, 1993·Toxicology in Vitro : an International Journal Published in Association with BIBRA·C L Bai, N H Stacey

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