PMID: 15360090Sep 14, 2004Paper

Comparison of DNA damage and repair following radiation challenge in buccal cells and lymphocytes using single-cell gel electrophoresis

International Journal of Radiation Biology
V S DhillonMichael Fenech

Abstract

To develop a reproducible single-cell gel electrophoresis assay for DNA damage and repair in buccal mucosa and sublingual exfoliated cells. Buccal mucosa and sublingual cells and lymphocytes from six individuals (three males, three females, aged 34-45 years) were challenged with increasing doses of gamma-rays. DNA strand breaks and DNA repair were measured using the single-cell gel electrophoresis assay. Baseline DNA strand breaks were significantly greater in buccal mucosa and sublingual cells compared with lymphocytes. Buccal mucosa and sublingual cells did not differ from each other with respect to induction of DNA strand breaks by 2 or 4 Gy gamma-rays. However, they showed a smaller increase in gamma-ray-induced DNA strand breaks compared with lymphocytes (32-53% less than lymphocytes; ANOVA p<0.0001). Unlike lymphocytes, which repaired 83% of DNA strand breaks, buccal mucosa and sublingual cells exhibited only a minimal capacity for DNA repair (approximately 0-14% of the level in lymphocytes). Buccal mucosa and sublingual cells exhibit an apparent resistance to the expression of radiation-induced DNA strand breaks in vitro and an apparent lack of DNA strand break repair in the single-cell gel electrophoresis assay.

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Citations

Jun 25, 2008·The British Journal of Nutrition·Siegfried KnasmüllerKarl-Heinz Wagner
Aug 1, 2012·Environmental and Molecular Mutagenesis·Fernanda Rabaioli Da SilvaKátia Kvitko
Jul 6, 2010·International Journal of Radiation Biology·Jorge E GonzálezLaurence Roy
Apr 4, 2014·Environmental Health Perspectives·Alessandro MarconRoberto de Marco
Nov 1, 2005·Environmental and Molecular Mutagenesis·Danillo PinhalDaisy Maria Favero Salvadori
Nov 7, 2019·Journal of Molecular Neuroscience : MN·Luciana Maria FelicianoDaisy Maria Fávero Salvadori
Mar 15, 2006·World Journal of Gastroenterology : WJG·Ling-Fei He, Jian-Gang Chen

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