Abstract
A dot Enzyme-linked Immunosorbent Assay (dot-ELISA), using whole cell Brucella abortus antigen dotted on the nitrocellulose membrane bound to a plastic strip (dipstick) was employed for the detection of Brucella antibodies in bovine sera. The results were compared with that of serum agglutination (SAT), Rose Bengal plate agglutination (RBPT) and Complement Fixation test (CFT). All the four tests gave negative reaction in 127 sera obtained from a brucellosis free herd. Testing of 549 sera from a chronically infected herd revealed 57 positive and 447 negative animals in all the four assays. Of the remaining 45 sera, 34 were positive in dot-ELISA. Six of these cases were independently detected by dot-ELISA while 28 showed positive reactions in combination with other tests. When serum samples from 158 aborted cases were subjected to dot-ELISA, 79 were found positive. Of these dot-ELISA positive cases, 71 gave positive reaction in SAT, 72 in RBPT and 78 in CFT. B. abortus biotype 3 was isolated from 34 of the 98 aborted fetuses examined.
References
Feb 1, 1975·Australian Veterinary Journal·G G AltonG G McPherson
May 1, 1976·Zentralblatt für Veterinärmedizin. Reihe B. Journal of veterinary medicine. Series B·H FeyF Grolimund
Jun 1, 1976·Australian Veterinary Journal·P J Mylrea, G C Fraser
Mar 1, 1976·Australian Veterinary Journal·P PlackettS J Best
Oct 24, 1985·Journal of Immunological Methods·S KumarG P Talwar
Jun 1, 1986·Veterinary Microbiology·M J LawmanM D Boyle
Jun 1, 1986·Veterinary Microbiology·S S Sutherland, L den Hollander
Dec 1, 1986·Australian Veterinary Journal·S S SutherlandJ Bathgate
Feb 13, 1988·The Veterinary Record·P ChandJ R Sadana
Jan 1, 1986·Methods in Enzymology·R Hawkes
Feb 1, 1985·Australian Veterinary Journal·C CargillI Clarke
Dec 1, 1980·The Journal of Hospital Infection·J D AbbottC Clifford
Sep 4, 1984·Journal of Immunological Methods·H Towbin, J Gordon
Jul 1, 1984·Veterinary Immunology and Immunopathology·M J LawmanM D Boyle
Dec 1, 1984·Veterinary Microbiology·A Van AertA Boeyé
Jan 1, 1982·Analytical Biochemistry·R HawkesJ Gordon
Citations
Oct 1, 1990·Veterinary Microbiology·R D VermaH V Batra
Sep 1, 1993·Veterinary Microbiology·P RamadassV D Padmanaban
Sep 9, 2005·Avian Pathology : Journal of the W.V.P.A·D MadhuriJ R Sadana
Oct 8, 1998·Veterinary Parasitology·F IbarraP Ochoa
Jun 1, 2010·Indian Journal of Microbiology·S MerwynG P Rai
May 22, 2002·Journal of Veterinary Medicine. B, Infectious Diseases and Veterinary Public Health·K GürtürkH Solmaz
Nov 1, 1992·World Journal of Surgery·M ImhofH D Röher
Jan 1, 1992·Intensive Care Medicine·C MadlK Lenz
Apr 11, 2006·Clinical and Vaccine Immunology : CVI·D GallP Smith