PMID: 9174139May 1, 1997Paper

Comparison of DRB sequence-based typing using different strategies

Tissue Antigens
Christina E M VoorterElla M van den Berg-Loonen

Abstract

Sequence-based typing (SBT) has become an important tool in the identification of HLA alleles. In this study a comparison was made between SBT of DRB1/3/4/5 alleles performed in two laboratories each using a different strategy for SBT. The laboratories in Utrecht and in Maastricht performed direct sequencing of PCR amplified genomic DNA from 30 selected samples. Primers and conditions for PCR amplification were different. Sequencing was either performed with T7 polymerase, using internal sequencing primers, or with cycle sequencing using an M13 tailed system. Two different automated DNA sequencers were used; the ALFexpress from Pharmacia and Applied Biosystems 373A. We concluded that nor the method of sequencing nor the sequencing machine influences typing results. However the PCR reaction used for generating template DNA is the most critical step. Different primers and different conditions can lead to false negative reactions. The fact that these false negative reactions can occur with different alleles in different combinations but not in all, implicates that extensive quality control is needed to assure correct typing results.

References

Feb 1, 1992·Human Immunology·P SantamariaS S Rich
Feb 11, 1988·Nucleic Acids Research·S A MillerH F Polesky
Jan 1, 1995·Tissue Antigens·P H SavelkoulE M van den Berg-Loonen
Aug 1, 1993·Human Immunology·E H RozemullerM G Tilanus
Jan 1, 1996·Tissue Antigens·E H RozemullerM G Tilanus

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Citations

May 19, 2001·Electrophoresis·J Drábek
Jun 7, 2005·Human Immunology·Wendy T N SwelsenElla M van den Berg-Loonen
Jun 7, 2005·Human Immunology·Wendy T N SwelsenElla M van den Berg-Loonen
Aug 23, 2005·Human Immunology·Christina E M VoorterElla M van den Berg-Loonen
Sep 17, 1999·Tissue Antigens·S A van der VliesE M van den Berg-Loonen
Mar 7, 2000·Tissue Antigens·C E VoorterE M van den Berg-Loonen
Jun 14, 2000·Tissue Antigens·D M DinauerM J Hessner
Dec 1, 2000·Tissue Antigens·C E VoorterE M van den Berg-Loonen
Dec 1, 2000·Tissue Antigens·C E VoorterE M van den Berg-Loonen
Sep 15, 2001·Tissue Antigens·C E VoorterE M van den Berg-Loonen
Oct 9, 2002·Tissue Antigens·C E M VoorterE M van den Berg-Loonen
Nov 26, 2002·Tissue Antigens·W T N SwelsenE M van den Berg-Loonen
Jan 25, 2003·Tissue Antigens·C E M VoorterE M van den Berg-Loonen
Oct 23, 1997·Tissue Antigens·C E VoorterE M van den Berg-Loonen
Dec 6, 1997·Tissue Antigens·C E VoorterE M van den Berg-Loonen
Feb 12, 1998·Tissue Antigens·C E VoorterE M van den Berg-Loonen
Jan 23, 1999·Tissue Antigens·S A van der VliesE M van den Berg-Loonen
Sep 10, 2010·Metabolism: Clinical and Experimental·Masanori MoriguchiHiroshi Ikegami
Dec 13, 2005·Annals of the New York Academy of Sciences·Giorgio La NasaFranco Locatelli
Jun 26, 2003·Tissue Antigens·W T N SwelsenE M van den Berg-Loonen
Mar 3, 2004·Tissue Antigens·W T N SwelsenE M van den Berg-Loonen
May 20, 2003·Tissue Antigens·C E M VoorterE M van den Berg-Loonen
Apr 24, 2004·Tissue Antigens·C E M VoorterE M van den Berg-Loonen
Apr 21, 2007·Tissue Antigens·C E M VoorterE M van den Berg-Loonen
Jun 15, 2007·Tissue Antigens·C E M VoorterE M van den Berg-Loonen
Sep 24, 2015·Tissue Antigens·K E H GerritsenM G J Tilanus
Feb 8, 2005·Human Immunology·Wendy T N SwelsenElla M van den Berg-Loonen
Sep 28, 2006·Human Immunology·Christina E M Voorter, Ella M van den Berg-Loonen
Jan 25, 2011·Human Immunology·Petal A WijnenMarjolein Drent
Feb 14, 2002·European Journal of Immunogenetics : Official Journal of the British Society for Histocompatibility and Immunogenetics·S VidalJ l Rodriguez-Sanchez

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