Comparison of droplet digital PCR and conventional quantitative PCR for measuring EGFR gene mutation

Experimental and Therapeutic Medicine
B O ZhangCong-Li Cai

Abstract

Early detection of epidermal growth factor receptor (EGFR) mutation, particularly EGFR T790M mutation, is of clinical significance. The aim of the present study was to compare the performances of amplification refractory mutation system-based quantitative polymerase chain reaction (ARMS-qPCR) and droplet digital polymerase chain reaction (ddPCR) approaches in the detection of EGFR mutation and explore the feasibility of using ddPCR in the detection of samples with low mutation rates. EGFR gene mutations in plasmid samples with different T790M mutation rates (0.1-5%) and 10 clinical samples were detected using the ARMS-qPCR and ddPCR approaches. The results demonstrated that the ARMS-qPCR method stably detected the plasmid samples (6,000 copies) with 5 and 1% mutation rates, while the ddPCR approach reliably detected those with 5% (398 copies), 1% (57 copies), 0.5% (24 copies) and 0.1% (average 6 copies) mutation rates. For the 10 clinical samples, the results for nine samples by the ARMS-qPCR and ddPCR methods were consistent; however, the sample N006, indicated to be EGFR wild-type by ARMS-qPCR, was revealed to have a clear EGFR T790M mutation with seven copies of mutant alleles in a background of 6,000 wild-type copies using ...Continue Reading

References

Mar 15, 2002·Seminars in Oncology·Wilbur A FranklinPaul A Bunn
May 16, 2003·Journal of Clinical Oncology : Official Journal of the American Society of Clinical Oncology·Masahiro FukuokaJosé Baselga
Jul 15, 2005·The New England Journal of Medicine·Frances A ShepherdUNKNOWN National Cancer Institute of Canada Clinical Trials Group
Jul 5, 2006·Clinical Cancer Research : an Official Journal of the American Association for Cancer Research·Hideharu KimuraKazuto Nishio
Nov 1, 2006·Journal of Clinical Oncology : Official Journal of the American Society of Clinical Oncology·Fred R HirschBrian Holloway
Feb 24, 2007·Nature Reviews. Cancer·Sreenath V SharmaDaniel A Haber
Sep 6, 2007·Clinical Cancer Research : an Official Journal of the American Association for Cancer Research·William Pao, Marc Ladanyi
Mar 12, 2009·Clinical Cancer Research : an Official Journal of the American Association for Cancer Research·Tony K F YungY M Dennis Lo
May 6, 2009·Journal of Clinical Oncology : Official Journal of the American Society of Clinical Oncology·Hua BaiJie Wang
Mar 12, 2011·Cancer Epidemiology, Biomarkers & Prevention : a Publication of the American Association for Cancer Research, Cosponsored by the American Society of Preventive Oncology·Yelena Y JanjigianManish A Shah
Jul 27, 2011·Journal of Clinical Oncology : Official Journal of the American Society of Clinical Oncology·Qing ZhouYi-Long Wu
Dec 13, 2012·International Journal of Cancer. Journal International Du Cancer·Bianca MostertJohn W M Martens
Mar 28, 2013·Clinical Cancer Research : an Official Journal of the American Association for Cancer Research·Hironobu ShigakiHideo Baba

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Citations

Mar 15, 2016·Lung Cancer : Journal of the International Association for the Study of Lung Cancer·Li-Yang ChenPan-Chyr Yang
Feb 20, 2016·Journal of Clinical Pathology·Umberto MalapelleGiancarlo Troncone
Dec 26, 2015·Biotechnology and Bioengineering·Elena CámaraPau Ferrer
Feb 2, 2017·International Journal of Molecular Sciences·Julie A VendrellJérôme Solassol
Mar 7, 2020·The American Journal of Surgical Pathology·Melanie C BoisJoseph J Maleszewski
Apr 28, 2020·Journal of Cancer Research and Clinical Oncology·Sameer A Dhayat, Zixuan Yang
Aug 16, 2016·Oncotarget·Emma E van der ToomKenneth J Pienta
Mar 13, 2016·Journal of Gastrointestinal Cancer·Jian ZhouXin-Rong Yang
Mar 7, 2021·Genes·Uršula Prosenc ZmrzljakAleksandra Nikolić
May 8, 2021·Monaldi Archives for Chest Disease = Archivio Monaldi Per Le Malattie Del Torace·Rajesh VenkataramAnirban Chakraborty
Sep 5, 2020·Transfusion Medicine and Hemotherapy : Offizielles Organ Der Deutschen Gesellschaft Fur̈ Transfusionsmedizin Und Immunham̈atologie·Marion EryilmazPeter Bugert

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