Comparison of effects induced by toxic applications of kainate and glutamate by glucose deprivation on area CA1 of rat hippocampal slices

Brain Research
K AliciU Heinemann

Abstract

Baseline and stimulus-induced changes in [Ca2+]o and [K+]o as well as field potentials (fp's) were studied during application of the excitatory amino acids kainate or glutamate, or during glucose deprivation in area CA1 and CA3 of rat hippocampal slices. Bath application of kainate in concentrations of 1, 2, 5, 8 and 10 mM induced a sudden rapid fall of [Ca2+]o in area CA1, associated with a negative shift of the slow fp. Kainate induced disappearance of stratum radiatum (SR) as well as alveus stimulation-evoked postsynaptic fp's, with partial recovery after application of up to 2 mM kainate, but no recovery after 5 mM kainate. Only afferent volleys and repetitive SR stimulation-induced decreases of [Ca2+]o recovered after 5 mM kainate. Similar observations were made with glutamate. Only when glutamate was applied with 20 mM, irreversible disappearance of postsynaptic fp's was noted. Glucose deprivation for 60-90 min led to an initial slow decline of [Ca2+]o in area CA1 and CA3, associated with increases in [K+]o, but no significant changes in the fp baseline. Before reaching the lowest level in [Ca2+]o, stimulation of afferent and efferent fibres in area CA1 and CA3 evoked epileptiform discharges. After reaching the lowest lev...Continue Reading

Citations

Mar 30, 2010·The European Journal of Neuroscience·Alexandra L Ferguson, Trevor W Stone
Dec 3, 2008·The European Journal of Neuroscience·Alexandra L Ferguson, Trevor W Stone
Apr 27, 2007·Neurobiology of Disease·Peter A AbdelmalikPeter L Carlen

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