PMID: 9433475Jan 20, 1998Paper

Comparison of eight commercial kits for quantitation of antineutrophil cytoplasmic antibodies (ANCA)

Journal of Immunological Methods
G WangW L Gross

Abstract

Antineutrophil cytoplasmic antibodies (ANCA) are used as diagnostic markers for systemic vasculitis. However, the specificity and sensitivity of ANCA detection differs from centre to centre due in large part to variations in methodology. We compared 8 commercial ELISA kits and an in-house method (HM) for their specificity and sensitivity in detecting ANCA against proteinase 3 (PR3-ANCA, 7 kits) and meyloperoxidse (MPO-ANCA, 8 kits). Sera from 5 patients with systemic lupus erythematosus (SLE), 28 with Wegener's granulomatosis (WG), 22 with microscopic polyangiitis (MPA), 5 with idiopathic rapidly progressive glomerulonephritis (RPGN), and 5 healthy controls were examined by both the indirect immunofluorescence technique (IFT) and the ELISA kits. Sera from healthy controls and patients with SLE or cANCA-negative WG were shown to be PR3-ANCA negative by all 7 PR3-ANCA kits. In 25 cANCA-positive sera from WG patients, PR3-ANCA positivity ranged from 44% to 84%. An absolute concordance among the 7 kits was noted in 56% of the cANCA-positive samples. The PR3-ANCA levels in 5 of the 7 kits correlated with the cANCA titers in IFT. Sera from the healthy controls and 4 out of the 5 SLE and pANCA-negative patients were found to be MPO-AN...Continue Reading

References

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Citations

Nov 26, 2003·Arthritis Care and Research : the Official Journal of the Arthritis Health Professions Association·J H StoneC L Burek
Jun 9, 2000·Kidney International·C F FranssenJ W Tervaert
Dec 16, 2003·Current Opinion in Rheumatology·Wilhelm H Schmitt, Fokko J van der Woude
Nov 24, 2004·Clinical Chemistry and Laboratory Medicine : CCLM·Danilo VillaltaSergio Stella
Jul 6, 2014·Journal of Immunology Research·Johannes Schulte-PelkumMichael Mahler
May 25, 2002·Clinical Immunology : the Official Journal of the Clinical Immunology Society·Kimberly A RussellUlrich Specks
Dec 20, 2007·American Journal of Clinical Pathology·Michelle TrevisinJudy Savige
Sep 29, 1998·Arthritis and Rheumatism·G S Hoffman, U Specks
Sep 18, 2009·Annals of the New York Academy of Sciences·Dirk RoggenbuckKarsten Conrad
Jun 27, 2013·Clinical Chemistry and Laboratory Medicine : CCLM·Antonella RadiceRenato A Sinico
Sep 3, 1999·Clinical and Diagnostic Laboratory Immunology·D Vassilopoulos, G S Hoffman

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