Comparison of eight routine unpublished LC-MS/MS methods for the simultaneous measurement of testosterone and androstenedione in serum

Clinica Chimica Acta; International Journal of Clinical Chemistry
Rahel M BüttlerAnnemieke C Heijboer

Abstract

Liquid-chromatography tandem mass spectrometry (LC-MS/MS) has become the method of choice in steroid hormone measurement. However, little information on the mutual agreement of LC-MS/MS methods is available. We compared eight routine unpublished LC-MS/MS methods for the simultaneous measurement of testosterone and androstenedione. Sixty random serum samples from male and female volunteers were analysed in duplicate by eight routine LC-MS/MS methods. We performed Passing-Bablok regression analyses and calculated Pearson's correlation coefficients to assess the agreement of the methods investigated with one published method known to be accurate. Intra-assay CV of each method was calculated from duplicate results, recoveries for each method were calculated from six spiked samples. Furthermore, a CV between the investigated methods was calculated. The concentrations ranged from 0.05-1.26 nmol/L, 6.15-24.44 nmol/L and 0.15-4.78 nmol/L for testosterone in females, testosterone in males and androstenedione, respectively. The intra-assay CVs were between 3.7-16.0%, 0.9-5.2% and 1.2-9.5% for testosterone in females, testosterone in males and androstenedione, respectively. The slopes of the regression lines ranged between 0.90-1.25, 0.87...Continue Reading

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Citations

Oct 14, 2017·Annals of Clinical Biochemistry·John W Honour
Jan 8, 2018·Clinical Chemistry and Laboratory Medicine : CCLM·Julie BrossaudKalyane Bach-Ngohou
Feb 14, 2018·Sexual Development : Genetics, Molecular Biology, Evolution, Endocrinology, Embryology, and Pathology of Sex Determination and Differentiation·Flaminia FanelliAntonio Balsamo
Apr 29, 2020·Annales d'endocrinologie·Jean-Benoît CorcuffJulie Brossaud
Jan 1, 2021·The Journal of Clinical Endocrinology and Metabolism·Jacquelien J HillebrandAnnemieke C Heijboer
May 8, 2021·Journal of Neuroendocrinology·Fernando Cázarez-MárquezAndries Kalsbeek
Jun 14, 2021·Clinica Chimica Acta; International Journal of Clinical Chemistry·Mariëtte T AckermansAnnemieke C Heijboer
Aug 31, 2021·Annals of Clinical Biochemistry·Brian G Keevil
Dec 10, 2017·Best Practice & Research. Clinical Endocrinology & Metabolism·Jonneke J HollandersMartijn J J Finken

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