Comparison of electrochemical detection of acetylcholine-induced nitric oxide release (NO) and contractile force measurement of rabbit isolated carotid artery endothelium

Journal of Pharmaceutical and Biomedical Analysis
Emrah KilincMehmet Ozsoz

Abstract

Since the identification of nitric oxide (NO) as an endothelial-derived relaxing factor, it became very important to quantify NO in biological models eventhough it is present in very low concentrations with a very short half-life. The use of electrochemistry as an alternative detection method is quite promising and electrochemical probes are now being developed to detect NO. This paper consists of an amperometric, bi-polymer modified, platinum-iridium microelectrode (Pt 90%-Ir 10% alloy, multistranded, total diameter 130 microm) design and its application for NO detection in acetylcholine (Ach) introduced, rabbit isolated carotid artery endothelium model. In a pH range of 3.0-10.0. pH 3.0 was found to be the optimum pH. As the pH values increased up to 10.0, the response current decreased as the oxidation of NO is catalyzed by H(+) in the acidic media. Temperature effect was checked at 25 degrees C (room temperature), 30 and 40 degrees C. An increasing trend was observed in sensor response with the increasing temperature. Most common biological interferences as ascorbic acid, uric acid and glucose were eliminated via bi-polymer coatings of four layers of Nafion and a layer of 50 mM o-phenylenediamine (OPD). When S/N ratio was a...Continue Reading

References

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Citations

Nov 26, 2002·Nitric Oxide : Biology and Chemistry·Tayfun DalbastiMehmet Ozsoz
Jul 16, 2009·Nitric Oxide : Biology and Chemistry·Catherine N Hall, John Garthwaite
Dec 11, 2020·Free Radical Research·Amr AliGuangdong Yang

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