PMID: 20644603Jul 21, 2010Paper

Comparison of enteropathogenic Escherichia coli (EPEC) diagnosis by serology and by polymerase chain reaction (PCR)

Revista de gastroenterología del Perú : órgano oficial de la Sociedad de Gastroenterología del Perú
Angela LluqueTheresa J Ochoa

Abstract

The identification of EPEC in clinical laboratories is based on the determination of the serotypes by agglutination with O and H antiserum. Currently the proper diagnosis of EPEC should be done by the identification of the intimin gen (eaeA) by PCR. To compare the diagnosis of EPEC by serotyping and by PCR. We collected EPEC strains, identify by their O antigen, from 4 clinical laboratories in Lima from diarrheal samples in children less than 5 years of age. In those strains we have searched for virulence genes by a real time multiplex PCR for the diarrheagenic E. coli. We collected 113 strains; 82% from children less than 2 years of age. Only 15 strains (13.3%) had the intimin gene and therefore a confirmatory diagnosis of EPEC. In addition we found 3 enterotoxigenic (ETEC), 3 shiga toxin-producing (STEC), 1 enteroagreggative (EAEC) and 1 enteroinvasive (EIEC) strains. PCR should be use for the proper identification of EPEC. However, molecular methods are still not easily available in clinical laboratories worldwide.

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