Comparison of enzymatic and antifungal properties between family 18 and 19 chitinases from S. coelicolor A3(2)

Bioscience, Biotechnology, and Biochemistry
Tomokazu KawaseTakeshi Watanabe

Abstract

Streptomyces coelicolor A3(2) has 13 chitinase genes encoding 11 family 18 and two family 19 chitinases. To compare enzymatic properties of family 19 chitinase and family 18 chitinases produced by the same organism, the four chitinases (Chi18bA, Chi18aC, Chi18aD, and Chi19F), whose genes are expressed at high levels in the presence of chitin, were produced in Escherichia coli and purified. The effect of pH on the hydrolytic activity was very different not only among the four chitinases but also among the substrates. The hydrolytic activity of Chi19F, family 19 chitinase, against soluble substrates was remarkably high as compared with three family 18 chitinases, but was the lowest against crystalline substrates among the four chitinases. On the contrary, Chi18aC, a family 18-subfamily A chitinase, showed highest activity against crystalline substrates. Only Chi19F exhibited significant antifungal activity. Based on these observations, the roles of family 19 chitinases are discussed.

References

Sep 26, 1986·Science·C Manoil, J Beckwith
May 18, 1981·Carbohydrate Research·H Yamada, T Imoto
Dec 15, 1994·Structure·A PerrakisC E Vorgias
Jan 1, 1993·The Plant Journal : for Cell and Molecular Biology·D B CollingeK Vad
Nov 1, 1995·Protein Science : a Publication of the Protein Society·C N PaceT Gray
May 16, 1998·Proceedings of the National Academy of Sciences of the United States of America·K A Brameld, W A Goddard
Dec 16, 1998·Science·UNKNOWN C. elegans Sequencing Consortium
Feb 5, 1999·Journal of Molecular Biology·J SugiyamaT Watanabe
Jun 11, 1999·Bioscience, Biotechnology, and Biochemistry·A SaitoK Miyashita
May 24, 2000·Proceedings of the National Academy of Sciences of the United States of America·D M van AaltenR K Wierenga
Aug 25, 2000·Acta Crystallographica. Section D, Biological Crystallography·M HahnW Höhne
Aug 2, 2001·Proceedings of the National Academy of Sciences of the United States of America·T M FinanA Pühler
Jun 6, 2003·Bioscience, Biotechnology, and Biochemistry·Yoshikane ItohYoko Nishizawa
Feb 10, 2004·Applied and Environmental Microbiology·Tomokazu KawaseTakeshi Watanabe

❮ Previous
Next ❯

Citations

Oct 15, 2013·Applied Microbiology and Biotechnology·Karin HjortSara Sjöling
Oct 11, 2012·Bioscience, Biotechnology, and Biochemistry·Tadayuki TorataniTakeshi Watanabe
Jul 25, 2009·Plant Molecular Biology·Wimal UbhayasekeraSherry L Mowbray
May 8, 2009·Bioscience, Biotechnology, and Biochemistry·Wei-Ming ChenShann-Tzong Jiang
Nov 2, 2007·Journal of Zhejiang University. Science. B·Xu-Fen ZhuJun-Li Feng
Feb 23, 2011·International Journal of Molecular Sciences·Kim-Chi HoangChun-Yi Liau
Apr 4, 2007·Applied Microbiology and Biotechnology·Hyun-Soo KimPeter N Golyshin
Jun 10, 2010·Applied Microbiology and Biotechnology·Jack H WongF Liu
May 1, 2012·Applied Microbiology and Biotechnology·Lakshmi PrasannaSigrid Gåseidnes
Feb 15, 2016·World Journal of Microbiology & Biotechnology·Benjamín Moreno CastilloGraciela Huerta Palacios
Jun 21, 2014·Environmental Microbiology·Yani BaiWietse de Boer
Sep 15, 2015·Bioscience, Biotechnology, and Biochemistry·Kazushi SuzukiTakeshi Watanabe
Mar 23, 2013·Microbiology·Rikki F FrederiksenJørgen J Leisner
May 7, 2014·Environmental Microbiology·Andrew C TolonenGeorge M Church
Mar 31, 2015·Bioscience, Biotechnology, and Biochemistry·Saki InamineToki Taira
Apr 22, 2015·International Journal of Biological Macromolecules·Lin ChenZiduo Liu
Aug 26, 2014·Pesticide Biochemistry and Physiology·Josefa BlayaMargarita Ros
Jun 3, 2011·FEMS Microbiology Ecology·Behnam NazariTakeshi Fujii
Feb 23, 2013·Bioresource Technology·Katta Suma, Appa Rao Podile
Dec 22, 2016·Marine Drugs·Sara Skøtt PaulsenHenrique Machado
Apr 4, 2015·FEMS Microbiology Letters·Pascal ViensRyszard Brzezinski
Jan 29, 2016·Scientific Reports·Xin-Yue ChanKok-Gan Chan
Jul 26, 2018·Bioscience Reports·Ayokunmi Oyeleye, Yahaya M Normi
Apr 10, 2016·Applied and Environmental Microbiology·Ayumi HoriuchiHaruyuki Atomi
May 2, 2018·The Journal of General and Applied Microbiology·Keitaro TakeMamoru Wakayama
Mar 8, 2008·Bioscience, Biotechnology, and Biochemistry·Ryoji MizunoTakeshi Watanabe
Jan 24, 2018·Applied Microbiology and Biotechnology·Stanley T C NguyenRenaud Berlemont
Jul 4, 2012·The Journal of Microbiology·Youssuf GherbawyGhada Khiralla

❮ Previous
Next ❯

Related Concepts

Related Feeds

Antifungals (ASM)

An antifungal, also known as an antimycotic medication, is a pharmaceutical fungicide or fungistatic used to treat and prevent mycosis such as athlete's foot, ringworm, candidiasis, cryptococcal meningitis, and others. Discover the latest research on antifungals here.

Antifungals

An antifungal, also known as an antimycotic medication, is a pharmaceutical fungicide or fungistatic used to treat and prevent mycosis such as athlete's foot, ringworm, candidiasis, cryptococcal meningitis, and others. Discover the latest research on antifungals here.