Comparison of enzymatic hydrolysis in a centrifugal partition chromatograph and stirred tank reactor

Journal of Chromatography. a
J Krause, J Merz

Abstract

Recently the Centrifugal Partition Chromatography (CPC) device is investigated as a reactor for biocatalytic reactions, as it enables biocatalyst immobilization without solid support and continuous operation of biphasic reaction systems. However, a detailed determination of the enzymes behavior in the CPC reactor and comparison to a classical stirred tank reactor (STR) for biphasic enzyme catalysis is not shown yet. In this study, the performance of an enzymatic biphasic hydrolysis reaction using lipase from Candida rugosa is systematically evaluated using a STR. The results are compared to different experiments conducted in the CPC reactor and used to evaluate the reaction performance in each. The same characteristics and limitations were observed in STR and CPC. At all states the CPC provided a similar reaction performance. However, the reaction in the CPC runs faster into limitations and was not easily scalable due to complex effects of the flow pattern. Although the enzyme was immobilized successfully and the activity of the lipase was preserved during CPC operation optimizations are needed to make the CPC reactor more competitive. For instance, scaling up the chamber geometry seems to be mandatory to increase the reaction ...Continue Reading

Citations

Jan 17, 2020·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Radu Claudiu FierascuElwira Sieniawska

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.