PMID: 3743553Jun 1, 1986

Comparison of enzyme-linked immunosorbent assay with coagglutination and latex agglutination for rapid diagnosis of pneumococcal pneumonia by detecting antigen in sputa

European Journal of Clinical Microbiology
H Holmberg, A Krook

Abstract

A sandwich enzyme-linked immunosorbent assay detecting the species-specific pneumococcal C polysaccharide was compared to latex agglutination and a coagglutination test which detected capsular pneumococcal antigens in sputum specimens with regard to specificity and sensitivity. Specimens from 52 patients with clinical and radiological evidence for pneumonia were tested. Twenty-one patients with Streptococcus pneumoniae isolated in sputum and 31 patients with a non-pneumococcal etiology were included. The predictive values for a positive test by enzyme-linked immunosorbent assay was 0.91 and for a negative test 0.97, by latex agglutination 0.90 and 0.91, and by coagglutination 0.84 and 0.85 respectively; these values did not show a statistically significant difference. Whereas agglutination tests are technically more simple and can be performed more rapidly, the enzyme-linked immunosorbent assay has the advantage of detecting pneumococcal C polysaccharide, an antigen common to all pneumococci. Thus it provides an interesting alternative to tests based on serum containing antibodies to all 83 different capsular polysaccharides.

References

Sep 1, 1979·Journal of Clinical Microbiology·S A HardingM A Sande
Aug 25, 1975·JAMA : the Journal of the American Medical Association·S B ThorsteinssonT Fagan
Dec 1, 1968·The Biochemical Journal·D E Brundish, J Baddiley
Apr 1, 1982·European Journal of Clinical Microbiology·M KalinE H Olausson

Citations

Mar 1, 1993·European Journal of Clinical Microbiology & Infectious Diseases : Official Publication of the European Society of Clinical Microbiology·W G BoersmaG H Koëter
Nov 1, 1989·European Journal of Clinical Microbiology & Infectious Diseases : Official Publication of the European Society of Clinical Microbiology·A OrtqvistA Krook
Jun 1, 1986·European Journal of Clinical Microbiology·A Balows
Feb 1, 1987·European Journal of Clinical Microbiology·S Lenthe-EboaF A Waldvogel
Jan 15, 1989·American Journal of Ophthalmology·W M SobolK R Wilhelmus
Nov 20, 2001·Chest·J DomínguezV Ausina
Jun 24, 2004·Pediatric Pulmonology·Altacílio A NunesMaria Tereza K Campos
Jan 30, 2014·Infection & chemotherapy·Joon Young SongMoon H Nahm
Jan 1, 1993·Scandinavian Journal of Infectious Diseases·Y HollowayJ A Snijder

Related Concepts

polysaccharide C-substance (Streptococcus)
Agglutination Tests
Antigens, Bacterial
Enzyme-Linked Immunosorbent Assay
Latex Fixation Tests
Streptococcal Pneumonia
Polysaccharides, Bacterial
Sputum, Induced
Streptococcus pneumoniae

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