Comparison of eosin and fluorescein conjugates for the photoinitiation of cell-compatible polymer coatings

PloS One
Jacob L LillyBrad J Berron

Abstract

Targeted photopolymerization is the basis for multiple diagnostic and cell encapsulation technologies. While eosin is used in conjunction with tertiary amines as a water-soluble photoinitiation system, eosin is not widely sold as a conjugate with antibodies and other targeting biomolecules. Here we evaluate the utility of fluorescein-labeled bioconjugates to photopolymerize targeted coatings on live cells. We show that although fluorescein conjugates absorb approximately 50% less light energy than eosin in matched photopolymerization experiments using a 530 nm LED lamp, appreciable polymer thicknesses can still be formed in cell compatible environments with fluorescein photosensitization. At low photoinitiator density, eosin allows more sensitive initiation of gelation. However at higher functionalization densities, the thickness of fluorescein polymer films begins to rival that of eosin. Commercial fluorescein-conjugated antibodies are also capable of generating conformal, protective coatings on mammalian cells with similar viability and encapsulation efficiency as eosin systems.

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Citations

Jan 25, 2019·Journal of Biological Engineering·Kara A DavisBrad J Berron
Jul 14, 2020·Macromolecular Rapid Communications·Nathan R B Boase
Jun 4, 2020·Journal of Biomedical Materials Research. Part a·Kara A DavisBrad J Berron
Jul 11, 2021·Acta Biomaterialia·Nathan GasekDaniel J Weiss
Jul 6, 2021·Macromolecular Rapid Communications·Sayeny de Ávila GonçalvesRoniérik Pioli Vieira

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Methods Mentioned

BETA
Chip
optical microscopy
Fluorescence

Software Mentioned

Array

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