Comparison of epithelial differentiation and immune regulatory properties of mesenchymal stromal cells derived from human lung and bone marrow.

PloS One
Mario RicciardiMauro Krampera

Abstract

Mesenchymal stromal cells (MSCs) reside in many organs including lung, as shown by their isolation from fetal lung tissues, bronchial stromal compartment, bronchial-alveolar lavage and transplanted lung tissues. It is still controversial whether lung MSCs can undergo mesenchymal-to-epithelial-transition (MET) and possess immune regulatory properties. To this aim, we isolated, expanded and characterized MSCs from normal adult human lung (lung-hMSCs) and compared with human bone marrow-derived MSCs (BM-hMSCs). Our results show that lung-MSCs reside at the perivascular level and do not significantly differ from BM-hMSCs in terms of immunophenotype, stemness gene profile, mesodermal differentiation potential and modulation of T, B and NK cells. However, lung-hMSCs express higher basal level of the stemness-related marker nestin and show, following in vitro treatment with retinoic acid, higher epithelial cell polarization, which is anyway partial when compared to a control epithelial bronchial cell line. Although these results question the real capability of acquiring epithelial functions by MSCs and the feasibility of MSC-based therapeutic approaches to regenerate damaged lung tissues, the characterization of this lung-hMSC populat...Continue Reading

References

May 1, 1976·Biulleten' eksperimental'noĭ biologii i meditsiny·Iu F GorskaiaN N Kulagins
Jan 1, 1995·Biology of the Neonate·H MasuyamaT Kudo
Jan 1, 1995·Annual Review of Biochemistry·A L Means, L J Gudas
Nov 11, 1999·Proceedings of the National Academy of Sciences of the United States of America·M A LaneL J Gudas
May 19, 2000·Cytokine & Growth Factor Reviews·P McCaffery, U C Dräger
Dec 6, 2000·The Journal of Clinical Investigation·Y KasaharaN F Voelkel
Jun 1, 2001·The Annals of Thoracic Surgery·A K KazaV E Laubach
Oct 19, 2001·The International Journal of Biochemistry & Cell Biology·J PeragónJ A Lupiáñez
Jan 25, 2002·Respiratory Research·Moisés Selman, Annie Pardo
Feb 26, 2003·American Journal of Respiratory Cell and Molecular Biology·Rubin M TuderSonia C Flores
May 15, 2003·Experimental Lung Research·Jacobien A NoordhoekJeannette F M van Straaten
Oct 17, 2003·Swiss Medical Weekly·Thomas Geiser
Aug 6, 2004·Philosophical Transactions of the Royal Society of London. Series B, Biological Sciences·Malcolm Maden, Matthew Hind
Oct 2, 2004·The European Respiratory Journal·O HolzR A Jörres
Nov 5, 2004·Cellular and Molecular Life Sciences : CMLS·C WieseA M Wobus
Dec 24, 2004·Proceedings of the National Academy of Sciences of the United States of America·Guoshun WangJay K Kolls
Mar 23, 2005·Biochemical and Biophysical Research Communications·Martin BrzoskaPatrick Baer
Jun 1, 2005·Laboratory Investigation; a Journal of Technical Methods and Pathology·Federica SabatiniDanièle Brouty-Boyé
Sep 24, 2005·American Journal of Respiratory and Critical Care Medicine·Roberto LoiDaniel J Weiss
Nov 1, 2005·American Journal of Physiology. Lung Cellular and Molecular Physiology·Chiharu TabataMichiaki Mishima
Apr 1, 2006·Journal of Cystic Fibrosis : Official Journal of the European Cystic Fibrosis Society·Massimo Conese, Joanna Rejman
Jun 17, 2006·Current Opinion in Pharmacology·Mauro KramperaFrancesco Annunziato
Oct 7, 2006·American Journal of Respiratory and Critical Care Medicine·Chiharu TabataHajime Kubo
Mar 10, 2007·The Journal of Clinical Investigation·Vibha N LamaVictor J Thannickal
Mar 4, 2008·The European Respiratory Journal·S Baraldo, M Saetta
May 10, 2008·American Journal of Respiratory and Critical Care Medicine·Shinsaku TogoStephen I Rennard
Jul 19, 2008·American Journal of Respiratory and Critical Care Medicine·Martina KorfeiAndreas Guenther
Sep 5, 2008·The Journal of Immunology : Official Journal of the American Association of Immunologists·Lamis JarvinenVibha N Lama
Sep 13, 2008·Cell Stem Cell·Mihaela CrisanBruno Péault
Jun 3, 2009·The Journal of Clinical Investigation·Hervé AcloqueM Angela Nieto
Jun 6, 2009·The New England Journal of Medicine·Manuel G CosioAlvar Agusti
Sep 4, 2009·The New England Journal of Medicine·Joaquin Lamela, Francisco Vega
Sep 11, 2009·Proceedings of the American Thoracic Society·William MacNee, Rubin M Tuder
Oct 24, 2009·American Journal of Respiratory and Critical Care Medicine·Thomas H SissonRichard H Simon

❮ Previous
Next ❯

Citations

Dec 20, 2013·Antioxidants & Redox Signaling·Jennifer J P Collins, Bernard Thébaud
Jul 31, 2013·Stem Cells and Development·Julia A PaxsonAndrew M Hoffman
Dec 29, 2013·Journal of Extracellular Vesicles·Soon Sim TanSai Kiang Lim
Feb 15, 2013·Respiration; International Review of Thoracic Diseases·Karthikeyan Ardhanareeswaran, Maria Mirotsou
Oct 4, 2013·Shock·Nuray Kusadasi, A B Johan Groeneveld
Dec 10, 2013·Translational Research : the Journal of Laboratory and Clinical Medicine·Kristen ThaneAndrew M Hoffman
Jul 9, 2013·Translational Research : the Journal of Laboratory and Clinical Medicine·Marco ChilosiVenerino Poletti
Apr 23, 2013·Cytotherapy·Mauro KramperaUNKNOWN MSC Committee of the International Society for Cellular Therapy (ISCT)
Oct 3, 2014·Respiratory Research·Mariana A AntunesPatricia R M Rocco
Aug 5, 2014·BMC Veterinary Research·Alice WongClare E Yellowley
Jan 16, 2013·Respirology : Official Journal of the Asian Pacific Society of Respirology·Kenneth SinclairDaniel C Chambers
Nov 24, 2012·BioEssays : News and Reviews in Molecular, Cellular and Developmental Biology·Michael D GreenHans-Willem Snoeck
Mar 13, 2014·Birth Defects Research. Part A, Clinical and Molecular Teratology·Jennifer J P Collins, Bernard Thébaud
Apr 3, 2014·IEEE Transactions on Bio-medical Engineering·Elizabeth A CalleLaura E Niklason
Aug 15, 2015·Stem Cells International·Natalia EscacenaAbdelkrim Hmadcha
Mar 11, 2015·ACS Applied Materials & Interfaces·Erika ZanchettaGiovanna Brusatin
May 3, 2018·Respiration; International Review of Thoracic Diseases·Dennis M L W KrukNick H Ten Hacken
Apr 13, 2019·American Journal of Respiratory Cell and Molecular Biology·Tamara CruzRosa Faner
Oct 31, 2019·Journal of Experimental & Clinical Cancer Research : CR·Mineko TeraoEnrico Garattini
Oct 10, 2019·Journal of Tissue Engineering and Regenerative Medicine·Pramod KumarAmir M Ghaemmaghami
Sep 8, 2016·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·Debashis HaldarPhilip N Newsome
Nov 13, 2015·Stem Cell Research & Therapy·Mahesh KhatriLinda J Saif
Jan 26, 2017·Frontiers in Endocrinology·Marjorie E BatemanBruce A Bunnell
May 14, 2020·American Journal of Physiology. Lung Cellular and Molecular Physiology·Stefanie Noel Sveiven, Tara M Nordgren

❮ Previous
Next ❯

Methods Mentioned

BETA
lavage
density gradient centrifugation
flow cytometry
PCR
electrophoresis
protein assay
FACS
SMA

Software Mentioned

Cell Quest

Related Concepts

Related Feeds

B cells: Gene Expression

B lymphocytes are white blood cells that play a role in the adaptive immune system by secreting antibodies. Here is the latest research on gene expression in B cells.

Cancer Biology: Molecular Imaging

Molecular imaging enables noninvasive imaging of key molecules that are crucial to tumor biology. Discover the latest research in molecular imaging in cancer biology in this feed.

Adult Stem Cells

Adult stem cells reside in unique niches that provide vital cues for their survival, self-renewal, and differentiation. They hold great promise for use in tissue repair and regeneration as a novel therapeutic strategies. Here is the latest research.

Cadherins and Catenins

Cadherins (named for "calcium-dependent adhesion") are a type of cell adhesion molecule (CAM) that is important in the formation of adherens junctions to bind cells with each other. Catenins are a family of proteins found in complexes with cadherin cell adhesion molecules of animal cells: alpha-catenin can bind to β-catenin and can also bind actin. β-catenin binds the cytoplasmic domain of some cadherins. Discover the latest research on cadherins and catenins here.

Aminoglycosides

Aminoglycoside is a medicinal and bacteriologic category of traditional Gram-negative antibacterial medications that inhibit protein synthesis and contain as a portion of the molecule an amino-modified glycoside. Discover the latest research on aminoglycoside here.

Allogenic & Autologous Therapies

Allogenic therapies are generated in large batches from unrelated donor tissues such as bone marrow. In contrast, autologous therapies are manufactures as a single lot from the patient being treated. Here is the latest research on allogenic and autologous therapies.

Aminoglycosides (ASM)

Aminoglycoside is a medicinal and bacteriologic category of traditional Gram-negative antibacterial medications that inhibit protein synthesis and contain as a portion of the molecule an amino-modified glycoside. Discover the latest research on aminoglycoside here.

Related Papers

Expert Opinion on Therapeutic Targets
Sheila A Doggrell
The New England Journal of Medicine
Joaquin Lamela, Francisco Vega
The New England Journal of Medicine
Richard C Boucher
© 2021 Meta ULC. All rights reserved