Comparison of evaluation techniques, including digital image analysis, for MYC protein expression by immunohistochemical stain in aggressive B-cell lymphomas

Human Pathology
Meghan HuppElizabeth L Courville

Abstract

Incorporation of an MYC immunohistochemical stain in the workup of large B-cell lymphomas has become common in hematopathology practice. Evaluation of this stain can be difficult because of staining heterogeneity and can have interobserver variability, particularly when performed on the entire tumor sections. We identified 87 cases of aggressive B-cell lymphoma (34 core needle and 53 excisional biopsies) and compared the following methods of MYC immunohistochemical staining evaluation: the original pathologist's interpretation, a systematic retrospective method of evaluation by manual analysis, and a retrospective method of evaluation by digital image analysis (using scanned slides analyzed via the Aperio Nuclear algorithm). Overall, concordance among these methods was around 80% with κ statistics showing good agreement. However, nearly one-third of our cases had a percent MYC positivity in the 30% to 50% range, and for these cases, concordance among the various methods was marginal/poor. This suggests limited utility as a prognostic or predictive marker using 40% as a cutoff value. In our series, core biopsy specimens were poor predictors of MYC gene rearrangement, and there was no association between MYC immunohistochemical s...Continue Reading

References

Feb 9, 2012·The American Journal of Surgical Pathology·Tina Marie GreenMichael Boe Møller
Apr 19, 2012·PloS One·Michael J KlukScott J Rodig
Jun 6, 2012·Journal of Clinical Oncology : Official Journal of the American Society of Clinical Oncology·Tina Marie GreenMichael Boe Møller
Aug 2, 2012·Journal of Clinical Oncology : Official Journal of the American Society of Clinical Oncology·Nathalie A JohnsonRandy D Gascoyne
Nov 20, 2012·Annual Review of Pathology·Farzad GhaznaviMichael Feldman
Jan 22, 2013·Blood·Heike HornUNKNOWN German High-Grade Non-Hodgkin Lymphoma Study Group
May 30, 2013·Haematologica·Alexandra ValeraUNKNOWN Grup per l’Estudi dels Limfomes de Catalunya i Balears (GELCAB)
Feb 11, 2014·British Journal of Haematology·Anamarija M PerryDennis D Weisenburger
Nov 29, 2014·Modern Pathology : an Official Journal of the United States and Canadian Academy of Pathology, Inc·Amer Z MahmoudMohammad A Vasef
Aug 5, 2015·Journal of Clinical Oncology : Official Journal of the American Society of Clinical Oncology·David W ScottRandy D Gascoyne
Feb 3, 2016·American Journal of Clinical Pathology·Michael J KlukScott J Rodig
May 31, 2017·Archives of Pathology & Laboratory Medicine·Famke AeffnerG Dave Young

❮ Previous
Next ❯

Related Concepts

Related Feeds

B-Cell Lymphoma

B-cell lymphomas include lymphomas that affect B cells. This subtype of cancer accounts for over 80% of non-Hodgkin lymphomas in the US. Here is the latest research.

© 2022 Meta ULC. All rights reserved