Comparison of extracellular enzyme activities and community composition of attached and free-living bacteria in porous medium columns.

Applied and Environmental Microbiology
R M Lehman, Seán P O'Connell

Abstract

Free-living and surface-associated microbial communities in sand-packed columns perfused with groundwater were compared by examination of compositional and functional characteristics. The composition of the microbial communities was assessed by bulk DNA extraction, PCR amplification of 16S ribosomal DNA fragments, separation of these fragments by denaturing gradient gel electrophoresis, and sequence analysis. Community function was assessed by measurement of beta-glucosidase and aminopeptidase extracellular enzyme activities. Free-living populations in the aqueous phase exhibited a greater diversity of phylotypes than populations associated with the solid phase. The attached bacterial community displayed significantly greater beta-glucosidase and aminopeptidase enzyme activities per volume of porous medium than those of the free-living community. On a per-cell basis, the attached community had a significantly higher cell-specific aminopeptidase enzyme activity (1.07 x 10(-7) nmol cell(-1) h(-1)) than the free-living community (5.02 x 10(-8) nmol cell(-1) h(-1)). Conversely, the free-living community had a significantly higher cell-specific beta-glucosidase activity (1.92 x 10(-6) nmol cell(-1) h(-1)) than the surface-associated...Continue Reading

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Citations

Jan 7, 2004·Journal of Microbiological Methods·A L Barkovskii, H Fukui
Oct 30, 2007·Applied and Environmental Microbiology·Hongyue DangGuiqiao Huang
Jan 11, 2005·Applied and Environmental Microbiology·Jutta KleikemperJosef Zeyer
Oct 6, 2005·Applied and Environmental Microbiology·Helen A VrionisDerek R Lovley
Apr 7, 2005·Applied and Environmental Microbiology·Daniel P ErwinRonald L Crawford
Feb 19, 2011·Annual Review of Marine Science·Carol Arnosti
Feb 11, 2015·The Science of the Total Environment·L WeaverM E Close
May 1, 2003·FEMS Microbiology Ecology·Seán P O'ConnellFrederick S Colwell
Oct 19, 2016·Environmental Science & Technology·Todd A OlsenScott C Brooks
Aug 24, 2018·FEMS Microbiology Ecology·Kosala A SirisenaGeoffrey K Chambers
Oct 5, 2010·Environmental Science & Technology·Christina N BrowHolly M Simon
May 24, 2008·Environmental Science & Technology·Yoshiko FujitaRobert W Smith

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