Abstract
This paper aims to define a robust procedure to extract extracellular polymeric substances (EPS) from aggregates of three benthic phototrophic microorganisms: the cyanobacterium Phormidium autumnale, the diatom Nitzschia palea, and the green alga Uronema confervicolum. This study focuses on the extraction efficiency of polysaccharide and protein EPS by using two physical methods (sonication, cation exchange resin) and three chemical methods (formamide, EDTA, Tween 20) with minimum cell lysis. Cell lysis was evaluated by monitoring chlorophyll a release. The results indicated that sonication or incubation of the algae aggregates with 0.25% Tween 20 induced a high level of cell lysis. A combined extraction approach, with an initial dispersing pretreatment (Ultra-Turrax, 13 500 r·min-1, 1 min), followed by formamide addition (0.22%) and then incubation with Dowex cation exchange resin (50 g per g of dry biomass), provided the highest amount of extracted EPS (mostly proteins), with low cell lysis. Furthermore, extracted EPS were characterized by size exclusion chromatography, and the obtained fingerprints revealed similar profiles for the three benthic microorganisms with a majority of low molecular weight polymers (400 to 11 300 D...Continue Reading
References
Oct 1, 1985·Analytical Biochemistry·P K SmithD C Klenk
Jul 1, 1996·Microbial Ecology·K Becker
Nov 18, 1997·Planta·J L LindR Wetherbee
Sep 8, 2001·Canadian Journal of Microbiology·J R LawrenceT R Neu
May 3, 2003·Water Research·Tatiana GörnerBruno S Lartiges
Sep 6, 2003·Applied and Environmental Microbiology·Tom J BattinClaude Hansen
Aug 2, 2006·Journal of Hazardous Materials·Sophie ComteMichel Baudu
Aug 15, 2006·Water Research·Martin PivokonskyLenka Pivokonska
Jan 17, 2007·Analytical and Bioanalytical Chemistry·Sergi SabaterMechthild Schmitt-Jansen
Mar 6, 2007·Applied and Environmental Microbiology·Francois AhimouPaige J Novak
Aug 7, 2007·Journal of Bacteriology·Hans-Curt FlemmingDaniel J Wozniak
Dec 11, 2007·Water Research·M RasD Lefebvre
Dec 29, 2007·Journal of Hazardous Materials·Sunil S Adav, Duu-Jong Lee
Sep 25, 2008·FEMS Microbiology Ecology·Joséphine LeflaiveLoïc Ten-Hage
May 21, 2009·FEMS Microbiology Ecology·Irene YllaSergi Sabater
Jun 13, 2009·FEMS Microbiology Ecology·Anne BöhmeKirsten Küsel
Jun 30, 2009·Environmental Pollution·Ai-Jun MiaoPeter H Santschi
Sep 4, 2009·Environmental Science and Pollution Research International·Sahlan Ozturk, Belma Aslim
Dec 22, 2009·Ecotoxicology·Alexandra SerraS A M Van Beusekom
Jul 27, 2010·Chemosphere·Zhiwei LiangPing Du
Aug 14, 2010·Biotechnology Advances·Guo-Ping ShengXiao-Yan Li
Nov 30, 2010·Biofouling·Veronica Diaz VillanuevaAnna M Romani
May 18, 2011·Bioresource Technology·Isabelle BourvenGilles Guibaud
Jan 3, 2012·Aquatic Toxicology·Anne-Sophie LambertStéphane Pesce
Jan 17, 2012·Applied and Environmental Microbiology·Jennifer K WeyMarkus Weitere
Apr 3, 2013·Carbohydrate Polymers·Kumari KavitaBhavanath Jha
Jun 22, 2013·FEMS Microbiology Ecology·Lone TangRikke L Meyer
Sep 1, 2014·Journal of Biotechnology·Xuesong GuoBenyi Xiao
Nov 26, 2014·Environmental Science and Pollution Research International·J LeflaiveM Danger
Mar 15, 2016·Nature Reviews. Microbiology·Tom J BattinAaron I Packmann
Jun 30, 2016·MBio·Rhona K StuartMichael P Thelen
Feb 19, 2017·Journal of Applied Microbiology·I Randrianjatovo-GbalouC-E Marcato-Romain
Jul 5, 2017·Journal of Biotechnology·Dorina StriethRoland Ulber