Comparison of extraction methods for the characterization of extracellular polymeric substances from aggregates of three biofilm-forming phototrophic microorganisms

Canadian Journal of Microbiology
Emilie LoustauElisabeth Girbal-Neuhauser

Abstract

This paper aims to define a robust procedure to extract extracellular polymeric substances (EPS) from aggregates of three benthic phototrophic microorganisms: the cyanobacterium Phormidium autumnale, the diatom Nitzschia palea, and the green alga Uronema confervicolum. This study focuses on the extraction efficiency of polysaccharide and protein EPS by using two physical methods (sonication, cation exchange resin) and three chemical methods (formamide, EDTA, Tween 20) with minimum cell lysis. Cell lysis was evaluated by monitoring chlorophyll a release. The results indicated that sonication or incubation of the algae aggregates with 0.25% Tween 20 induced a high level of cell lysis. A combined extraction approach, with an initial dispersing pretreatment (Ultra-Turrax, 13 500 r·min-1, 1 min), followed by formamide addition (0.22%) and then incubation with Dowex cation exchange resin (50 g per g of dry biomass), provided the highest amount of extracted EPS (mostly proteins), with low cell lysis. Furthermore, extracted EPS were characterized by size exclusion chromatography, and the obtained fingerprints revealed similar profiles for the three benthic microorganisms with a majority of low molecular weight polymers (400 to 11 300 D...Continue Reading

References

Oct 1, 1985·Analytical Biochemistry·P K SmithD C Klenk
Sep 8, 2001·Canadian Journal of Microbiology·J R LawrenceT R Neu
Sep 6, 2003·Applied and Environmental Microbiology·Tom J BattinClaude Hansen
Aug 2, 2006·Journal of Hazardous Materials·Sophie ComteMichel Baudu
Jan 17, 2007·Analytical and Bioanalytical Chemistry·Sergi SabaterMechthild Schmitt-Jansen
Mar 6, 2007·Applied and Environmental Microbiology·Francois AhimouPaige J Novak
Aug 7, 2007·Journal of Bacteriology·Hans-Curt FlemmingDaniel J Wozniak
Dec 11, 2007·Water Research·M RasD Lefebvre
Dec 29, 2007·Journal of Hazardous Materials·Sunil S Adav, Duu-Jong Lee
Sep 25, 2008·FEMS Microbiology Ecology·Joséphine LeflaiveLoïc Ten-Hage
May 21, 2009·FEMS Microbiology Ecology·Irene YllaSergi Sabater
Jun 13, 2009·FEMS Microbiology Ecology·Anne BöhmeKirsten Küsel
Jun 30, 2009·Environmental Pollution·Ai-Jun MiaoPeter H Santschi
Sep 4, 2009·Environmental Science and Pollution Research International·Sahlan Ozturk, Belma Aslim
Jan 17, 2012·Applied and Environmental Microbiology·Jennifer K WeyMarkus Weitere
Nov 26, 2014·Environmental Science and Pollution Research International·J LeflaiveM Danger
Mar 15, 2016·Nature Reviews. Microbiology·Tom J BattinAaron I Packmann
Jul 5, 2017·Journal of Biotechnology·Dorina StriethRoland Ulber

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Citations

Nov 5, 2019·Environmental Science and Pollution Research International·Emilie LoustauJean-Luc Rols
Mar 8, 2020·Water Science and Technology : a Journal of the International Association on Water Pollution Research·Ziyi Liu, Stephen R Smith

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